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Updated: Apr 30, 2026

Cell Labeling and Injection in Developing Embryonic Mouse Hearts

Cell Labeling and Injection in Developing Embryonic Mouse Hearts

Published on: April 17, 2014

Cell labeling and injection in developing embryonic mouse hearts.

Emilye Hiriart¹, Patrick van Vliet², Ralf J Dirschinger²

¹INSERM UMR-910, Aix-Marseille University.

Journal of Visualized Experiments : Jove

|May 7, 2014

Summary

Researchers developed new microinjection techniques to track stem cell development in mouse embryos. This method allows for better understanding of cell fate within the natural embryonic environment, improving developmental biology research.

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Area of Science:

Developmental Biology
Stem Cell Biology
Regenerative Medicine

Background:

In vitro studies of stem cell derivatives often yield controversial results, not reflecting their true in vivo potential.
Current cell lineage tracing methods in mouse embryos are limited to early developmental stages with underdeveloped organs.

Purpose of the Study:

To develop advanced microinjection techniques for studying cell fate in later-stage mouse embryos.
To enable precise delivery of various agents into targeted embryonic organs, including the heart.
To overcome limitations of current methods for in vivo developmental studies.

Main Methods:

Designed standard and ultrasound-mediated microinjection protocols for mouse embryos at embryonic day 9.5 and later.
Injected agents such as fluorescent dyes, viruses, shRNAs, and stem cell-derived progenitors into targeted cardiac regions.

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Cell Labeling and Injection in Developing Embryonic Mouse Hearts

Cell Labeling and Injection in Developing Embryonic Mouse Hearts

Published on: April 17, 2014

A Method for Labeling Vasculature in Embryonic Mice

A Method for Labeling Vasculature in Embryonic Mice

Published on: October 7, 2011

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

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Published on: April 17, 2019

Utilized embryonic explant cultures or in utero development to monitor cell behavior.

Main Results:

Successfully preserved organ function during the injection and monitoring process.
Enabled effective tracking of the migration and fate of labeled and injected cells within the developing embryonic heart.
Demonstrated the feasibility of studying cell behavior in a more physiologically relevant embryonic environment.

Conclusions:

The developed microinjection technologies provide a robust method for studying cell fate and migration in vivo.
These techniques overcome previous limitations, allowing for research in later developmental stages and more complex organ systems.
The approach holds significant potential for advancing key biological questions in developmental biology and regenerative medicine across various organs.