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Updated: Apr 30, 2026
Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation
Published on: July 6, 2019
Pleurotus eryngii Ameliorates Lipopolysaccharide-Induced Lung Inflammation in Mice
Junya Kawai1, Tsugunobu Andoh2, Kenji Ouchi1
1Mushroom Research Laboratory, Hokuto Corporation, 800-8 Shimokomazawa, Nagano 381-0008, Japan.
Abstract:
Pleurotus eryngii (P. eryngii) is consumed as a fresh cultivated mushroom worldwide and demonstrated to have multiple beneficial effects. We investigated the anti-inflammatory effect of P. eryngii in mice with acute lung injury (ALI). Intranasal instillation of lipopolysaccharide (LPS) (10 μ g/site/mouse) induced marked lung inflammation (increase in the number of inflammatory cells, protein leakage, and production of nitric oxide in bronchoalveolar lavage fluid) as well as histopathological damage in the lung, 6 h after treatment. Mice administered heat-treated P. eryngii (0.3-1 g/kg, p.o. (HTPE)) 1 h before LPS challenge showed decreased pulmonary inflammation and ameliorated histopathological damage. These results suggest that HTPE has anti-inflammatory effects against ALI. Thus, P. eryngii itself may also have anti-inflammatory effects and could be a beneficial food for the prevention of ALI induced by bacterial infection.
Insights
Heat-treated Pleurotus eryngii (HTPE) demonstrates anti-inflammatory effects against acute lung injury (ALI) in mice. This suggests P. eryngii could be a beneficial food for preventing bacterial-induced ALI.
Area of Science:
- Mushroom research
- Immunology
- Pulmonary medicine
Background:
- Pleurotus eryngii (P. eryngii) is a widely consumed mushroom with known beneficial effects.
- Acute lung injury (ALI) is a severe inflammatory condition affecting the lungs.
Purpose of the Study:
- To investigate the anti-inflammatory effects of P. eryngii on lipopolysaccharide (LPS)-induced ALI in a mouse model.
- To determine if heat-treated P. eryngii (HTPE) can mitigate lung inflammation and damage.
Main Methods:
- Mice were induced with ALI using intranasal lipopolysaccharide (LPS).
- Mice were administered heat-treated P. eryngii (HTPE) orally one hour before LPS challenge.
- Pulmonary inflammation markers (inflammatory cells, protein, nitric oxide) and lung histopathology were assessed.
Main Results:
- LPS instillation caused significant lung inflammation and histopathological damage.
- HTPE administration significantly decreased pulmonary inflammation.
- HTPE treatment ameliorated the histopathological damage in the lungs.
Conclusions:
- Heat-treated P. eryngii (HTPE) exhibits significant anti-inflammatory effects against acute lung injury.
- P. eryngii possesses inherent anti-inflammatory properties.
- P. eryngii may serve as a beneficial dietary component for preventing bacterial infection-induced ALI.
