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[Efficiency of three methods for detecting Toxoplasma IgG antibody]
Objective:
To discuss the test efficiency of three methods for detecting Toxoplasma IgG antibody.
Methods:
Totally 304 specimens were detected parallelly for Toxoplasma IgG antibody by using the gold marked method, indirect hemagglutination test (IHA), and enzyme-linked immunosorbent assay (ELISA), and the sensitivity, specificity and Youden index of these methods were compared.
Results:
The detection sensitivities of gold marked method, IHA, and ELISA for Toxoplasma IgG antibody were 85.5%, 89.8% and 91.9% respectively (chi2 = 4.12, P > 0.05); the specificities were 92.4%, 96.6% and 97.5% respectively (chi2 = 4.06, P > 0.05). The detection efficiency and Youden index of ELISA were 94.1% and 0.89 respectively, being higher than those of IHA and gold marked method.
Conclusion:
The sensitivity and specificity of the ELISA method for Toxoplasma IgG antibody are higher, and in addition, it can be automated. Therefore, it is suitable for large-scale Toxoplasma IgG antibody screening.
