Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Translesion DNA Polymerases02:10

Translesion DNA Polymerases

9.3K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
9.3K
The Replisome03:01

The Replisome

31.2K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
31.2K
Mismatch Repair01:36

Mismatch Repair

38.1K
Overview
38.1K
Mismatch Repair01:20

Mismatch Repair

5.4K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
5.4K
Proofreading01:31

Proofreading

7.6K
Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase...
7.6K
Proofreading01:43

Proofreading

52.0K
Overview
52.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Revisiting the Conformational Flexibility of DNA 3-Arm Junctions for Nanoconstruction.

Nano letters·2026
Same author

Beyond Isolated Optimization: A Holistic Review Across the Pre-Mid Post-Treatment Chain for Hard Carbon in Sodium-Ion Battery.

Nano-micro letters·2026
Same author

Assembly of Protein-DNA Framework Nanostructures: Structurally Defining Protein-DNA Interfaces With Aptamer.

Angewandte Chemie (International ed. in English)·2026
Same author

Uric acid promotes dietary lipid absorption through microbiome and metabolomic remodeling via a liver-gut endocrine axis.

Cell host & microbe·2026
Same author

YY1 Lactylation Elicits CARD9 Deficiency in Dendritic Cells Promoting Pancreatic Cancer Immune Escape.

International journal of biological sciences·2026
Same author

Baicalein suppresses mitochondrial biogenesis via the RBM43/PGC-1α axis in triple-negative breast cancer cells.

Tissue & cell·2026

Related Experiment Video

Updated: Apr 30, 2026

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
08:00

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

6.6K

Vernier assembly: controlling DNA polymerization via length mismatching.

Xiang Li1, Chenhui Hao, Cheng Tian

  • 1Department of Chemistry, Purdue University, West Lafayette, IN, USA. mao@purdue.edu.

Chemical Communications (Cambridge, England)
|May 8, 2014
PubMed
Summary
This summary is machine-generated.

Scientists precisely controlled DNA polymer lengths using a Vernier scale-inspired method. This length mismatching technique offers new possibilities for designing complex DNA nanostructures.

More Related Videos

Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

6.7K
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.2K

Related Experiment Videos

Last Updated: Apr 30, 2026

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers
08:00

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

6.6K
Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

6.7K
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

1.2K

Area of Science:

  • Biochemistry
  • Nanotechnology
  • Molecular Biology

Background:

  • Supramolecular DNA polymers are essential for nanotechnology.
  • Precise control over polymer length is crucial for predictable self-assembly.

Purpose of the Study:

  • To develop a novel method for controlling supramolecular DNA polymer lengths.
  • To adapt the Vernier scale principle for molecular assembly.

Main Methods:

  • Utilized length mismatching between DNA strands.
  • Applied the Vernier scale principle to guide polymerization.
  • Characterized DNA polymer lengths using gel electrophoresis and microscopy.

Main Results:

  • Successfully controlled the lengths of supramolecular DNA polymers with high precision.
  • Demonstrated a scalable method for length control.
  • Enabled the creation of DNA nanostructures with defined dimensions.

Conclusions:

  • Length mismatching is an effective strategy for precise DNA polymer length control.
  • This method advances the design of complex DNA-based nanomaterials.
  • Opens avenues for programmable molecular construction.