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Updated: Apr 30, 2026
06:34
Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
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Paludibacterium paludis sp. nov., isolated from a marsh
Shih-Yi Sheu1, Zih-Han Chen2, Chiu-Chung Young3
1Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Summary
A novel bacterium, Paludibacterium paludis sp. nov. (KBP-21(T)), was identified from Taiwan
Area of Science:
- Microbiology
- Bacterial Taxonomy
- Environmental Microbiology
Background:
- The genus Paludibacterium belongs to the family Neisseriaceae within the class Betaproteobacteria.
- Understanding bacterial diversity in aquatic environments is crucial for ecological studies.
Purpose of the Study:
- To characterize a novel bacterial strain, KBP-21(T), isolated from a Taiwanese wetland.
- To determine the taxonomic position of strain KBP-21(T) within the genus Paludibacterium.
Main Methods:
- Polyphasic taxonomic approach including phenotypic, genotypic, and chemotaxonomic analyses.
- 16S rRNA gene sequencing for phylogenetic analysis.
- Analysis of cellular fatty acids, respiratory quinones, and polar lipids.
Main Results:
- Strain KBP-21(T) are Gram-negative, facultatively anaerobic, motile rods forming yellow colonies.
- Phylogenetic analysis placed strain KBP-21(T) as a member of the genus Paludibacterium, with Paludibacterium yongneupense 5YN8-15(T) as the closest neighbor (96.4% 16S rRNA gene similarity).
- Predominant fatty acids include summed feature 3, C16:0, and C18:1ω7c; major quinone is Q-8; DNA G+C content is 62.1 mol%.
Conclusions:
- Based on comprehensive data, strain KBP-21(T) represents a new species within the genus Paludibacterium.
- The proposed name for this novel species is Paludibacterium paludis sp. nov., with KBP-21(T) as the type strain.
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