iMatrixSpray: a free and open source sample preparation device for mass spectrometric imaging.

Area of Science:

• Analytical Chemistry
• Instrumentation
• Mass Spectrometry

Background:

• Matrix deposition is crucial for mass spectrometric imaging (MSI).
• Existing methods may require significant user interaction or lack robustness.
• A need exists for automated and accessible matrix deposition solutions.

Purpose of the Study:

• To develop an automated, user-friendly device for matrix deposition in MSI.
• To create a robust and adaptable instrument suitable for broad scientific adoption.
• To provide open-source hardware and software for the developed device.

Main Methods:

• A spray-type instrument utilizing delta-robotics for precise movement.
• Integration of a simple liquid handling system for matrix solution delivery.
• Development of pre-defined and custom method selection for user convenience.
• Open-source hardware and software design principles were followed.

Main Results:

• The device automates matrix deposition, minimizing user intervention.
• The delta-robotics design ensures instrument robustness and reliability.
• The open-source nature facilitates widespread distribution and community adaptation.
• The system is suitable for both pre-defined and custom matrix application methods.

Conclusions:

• The developed spray-type device offers an automated, robust, and accessible solution for matrix deposition in MSI.
• Its open-source design promotes collaboration and innovation within the scientific community.
• This instrument has the potential to streamline MSI workflows and enhance data quality.