Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chiral plasmonic Cu<sub>2-x</sub>S quantum dots enable chirality-dependent fluorescent recognition of tryptophan enantiomers.

Talanta·2026
Same author

Effects of pediatric cataract surgery on the axial length/corneal radius ratio and choroidal thickness in school-age children: a prospective cohort study.

International journal of ophthalmology·2026
Same author

Assembly of Protein-DNA Framework Nanostructures: Structurally Defining Protein-DNA Interfaces With Aptamer.

Angewandte Chemie (International ed. in English)·2026
Same author

AXOLOTL: an accurate method for detecting aberrant gene expression in rare diseases using coexpression constraints.

Bioinformatics (Oxford, England)·2026
Same author

The KXD1-TSPAN14 axis controls megakaryopoiesis and platelet production via Notch signaling.

Science China. Life sciences·2026
Same author

Fluorescence Imaging Analysis of PTK7 Clustering in Situ via a Programmable DNA Network for Apoptosis Induction and Drug Resistance Reversal.

Analytical chemistry·2026

Related Experiment Video

Updated: Apr 30, 2026

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.2K

One-step prepared fluorescent copper nanoclusters for reversible pH-sensing.

Wei Wang1, Fei Leng, Lei Zhan

  • 1Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, P. R. China. chengzhi@swu.edu.cn.

The Analyst
|May 8, 2014
PubMed
Summary

Researchers developed stable, water-soluble fluorescent copper nanoclusters (CuNCs) using trypsin in a single step. These pH-responsive CuNCs show excellent resistance to oxidation, heat, and light.

More Related Videos

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles
12:51

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

9.4K
Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
09:11

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Published on: March 22, 2020

7.2K

Related Experiment Videos

Last Updated: Apr 30, 2026

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.2K
A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles
12:51

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

9.4K
Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
09:11

Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications

Published on: March 22, 2020

7.2K

Area of Science:

  • Nanotechnology
  • Biochemistry
  • Materials Science

Background:

  • Developing stable fluorescent nanomaterials is crucial for various applications.
  • Copper nanoclusters (CuNCs) offer unique optical properties but often require complex stabilization methods.

Purpose of the Study:

  • To report a facile one-step synthesis of water-soluble and pH-responsive fluorescent copper nanoclusters (CuNCs).
  • To investigate the stability of these trypsin-stabilized CuNCs.

Main Methods:

  • A one-step synthesis approach was employed.
  • Trypsin was used as a stabilizing agent for the copper nanoclusters.
  • Characterization of the synthesized CuNCs was performed.

Main Results:

  • Water-soluble and pH-responsive fluorescent CuNCs were successfully synthesized in one step.
  • The as-prepared CuNCs demonstrated remarkable stability, including resistance to oxidation, thermal degradation, and photobleaching.
  • No additional protective or reducing agents were required during synthesis.

Conclusions:

  • A straightforward and efficient method for preparing highly stable fluorescent copper nanoclusters was established.
  • Trypsin stabilization provides excellent protective properties for CuNCs, enhancing their applicability.
  • The synthesized CuNCs are promising for applications requiring robust fluorescent probes.