Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mitochondrial Membranes01:45

Mitochondrial Membranes

11.7K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
11.7K
Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

4.5K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.5K
The Inner Mitochondrial Membrane01:28

The Inner Mitochondrial Membrane

3.8K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria.  In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
3.8K
Stem Cell Niche01:26

Stem Cell Niche

5.0K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.0K
Mitochondria01:37

Mitochondria

13.4K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
13.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gut-brain axis in Alzheimer's disease: neural and immune circuits linking peripheral dysbiosis to neurodegeneration.

Current opinion in neurobiology·2026
Same author

Tryptophan-kynurenine metabolic reprogramming along the gut-brain axis alleviates Alzheimer's pathology.

Journal of neuroinflammation·2026
Same author

Human brain and organoid transcriptomes reveal key receptor tyrosine kinase pathways and genetic signatures in Alzheimer's disease.

Experimental & molecular medicine·2026
Same author

Dual Targeting of Tau Kinases and Autophagy by Abemaciclib Independent of CDK4/6 Inhibition.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Establishment of Dementia Platform Korea and the Trial Ready Registry to advance Alzheimer's disease research in Korea.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same author

Basic Science and Pathogenesis.

Alzheimer's & dementia : the journal of the Alzheimer's Association·2025
Same journal

A transferrin receptor-based vector enables robust Type-II membrane protein display on mammalian cells.

Biochemical and biophysical research communications·2026
Same journal

NAT10-mediated RNA N4-acetylation promotes intestinal fibroblast senescence via DHRS2.

Biochemical and biophysical research communications·2026
Same journal

Deciphering potent MPL activation by the fucose-binding lectin thrombocorticin.

Biochemical and biophysical research communications·2026
Same journal

Mitochondrial genome alterations in cancer: From mutations and epigenetics to targeted therapiesack.

Biochemical and biophysical research communications·2026
Same journal

GPC3 chimeric antigen receptor (CAR)-NK cells combined with Enoblituzumab enhance the anti-tumor efficacy against hepatocellular carcinoma.

Biochemical and biophysical research communications·2026
Same journal

A miR-382-5p-PORCN axis modulates endogenous Wnt signaling during palatal development.

Biochemical and biophysical research communications·2026
See all related articles

Related Experiment Video

Updated: Apr 30, 2026

Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps
07:17

Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps

Published on: July 30, 2019

7.5K

Modulation of mitochondrial function by stem cell-derived cellular components.

Tian Liu1, Wooseok Im2, Soon-Tae Lee2

  • 1Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Republic of Korea.

Biochemical and Biophysical Research Communications
|May 8, 2014
PubMed
Summary
This summary is machine-generated.

Human adipose stem cell extracts protect against Huntington's disease (HD) by preventing neuronal death and improving mitochondrial function. These extracts reduce toxicity and apoptosis by targeting the p53 pathway, offering a potential therapeutic strategy for HD.

Keywords:
ApoptosisHuman adipose stem cellsMitochondriaMutant huntingtinp53

More Related Videos

Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells
12:42

Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells

Published on: June 17, 2008

14.1K
Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer
10:17

Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer

Published on: September 24, 2021

2.5K

Related Experiment Videos

Last Updated: Apr 30, 2026

Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps
07:17

Improving the Accuracy of Flow Cytometric Assessment of Mitochondrial Membrane Potential in Hematopoietic Stem and Progenitor Cells Through the Inhibition of Efflux Pumps

Published on: July 30, 2019

7.5K
Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells
12:42

Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells

Published on: June 17, 2008

14.1K
Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer
10:17

Assessment of Cellular Bioenergetics in Mouse Hematopoietic Stem and Primitive Progenitor Cells using the Extracellular Flux Analyzer

Published on: September 24, 2021

2.5K

Area of Science:

  • Neuroscience
  • Stem Cell Biology
  • Genetics

Background:

  • Huntington's disease (HD) is a hereditary neurodegenerative disorder characterized by striatal neuron loss due to mutant huntingtin protein (mHtt) aggregation.
  • Previous research indicates human adipose stem cells (hASC) and their extracts can mitigate HD progression in vitro and in vivo.
  • hASC are accessible adult stem cells, and their cytosolic extracts contain beneficial neurotrophic factors.

Purpose of the Study:

  • To investigate the protective effects of hASC extracts on neuronal death and mitochondrial function in the context of HD.
  • To elucidate the molecular mechanisms underlying the therapeutic potential of hASC extracts in HD.

Main Methods:

  • Cellular assays to assess toxicity and apoptosis induced by mHtt.
  • Mitochondrial function analysis, including oxidative stress and membrane potential measurements.
  • Western blotting and gene expression analysis to evaluate p53 levels and interactions.

Main Results:

  • hASC extracts effectively prevented mHtt-induced cell toxicity and apoptosis.
  • Extracts restored mitochondrial function by reducing oxidative stress and normalizing membrane potential.
  • hASC extracts inhibited p53-mHtt interaction and decreased p53 levels, leading to increased neuronal survival.

Conclusions:

  • hASC extracts exhibit protective effects against mHtt-induced mitochondrial apoptosis in HD models.
  • The mechanism involves the modulation of the p53 pathway, highlighting its therapeutic relevance.
  • These findings support the use of hASC extracts as a potential therapeutic strategy for Huntington's disease.