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Updated: Apr 30, 2026
Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts
Published on: April 26, 2018
A dual functional fluorescent probe for glioma imaging mediated by blood-brain barrier penetration and glioma cell
Hongwei Ma1, Zhiyong Gao2, Panfeng Yu3
1Department of Neurosurgery, Chinese PLA General Hospital, No. 28 Fuxing Road, Beijing 100853, PR China; School of Pharmacy, Fudan University, No. 826 Zhangheng Road, Shanghai 201203, PR China.
Abstract:
Glioma is a huge threat for human being because it was hard to be completely removed owing to both the infiltrating growth of glioma cells and integrity of blood brain barrier. Thus effectively imaging the glioma cells may pave a way for surgical removing of glioma. In this study, a fluorescent probe, Cy3, was anchored onto the terminal of AS1411, a glioma cell targeting aptamer, and then TGN, a BBB targeting peptide, was conjugated with Cy3-AS1411 through a PEG linker. The production, named AsT, was characterized by gel electrophoresis, (1)H NMR and FTIR. In vitro cellular uptake and glioma spheroid uptake demonstrated the AsT could not only be uptaken by both glioma and endothelial cells, but also penetrate through endothelial cell monolayer and uptake by glioma spheroids. In vivo, AsT could effectively target to glioma with high intensity. In conclusion, AsT could be used as an effective glioma imaging probe.
Insights
This study developed AsT, a novel fluorescent probe for glioma imaging. AsT effectively targets and illuminates glioma cells, aiding in surgical removal and improving patient outcomes.
Area of Science:
- Biomedical Engineering
- Molecular Imaging
- Cancer Research
Background:
- Glioma presents a significant challenge due to infiltrating cells and the blood-brain barrier (BBB).
- Effective imaging is crucial for complete surgical resection of gliomas.
- Current imaging techniques face limitations in targeting and visualizing glioma cells within the brain.
Purpose of the Study:
- To develop and characterize a novel fluorescent probe for enhanced glioma cell imaging.
- To evaluate the probe's ability to target glioma cells and penetrate the blood-brain barrier.
- To assess the probe's efficacy for in vivo glioma imaging.
Main Methods:
- Synthesis of AsT: Cy3 fluorescent probe conjugated with AS1411 aptamer and TGN peptide via a PEG linker.
- Characterization of AsT using gel electrophoresis, 1H NMR, and FTIR.
- In vitro studies: Cellular and glioma spheroid uptake assays.
- In vivo studies: Glioma targeting and imaging in animal models.
Main Results:
- AsT was successfully synthesized and characterized.
- In vitro studies confirmed AsT uptake by glioma and endothelial cells, with penetration through endothelial monolayers.
- AsT demonstrated effective targeting and high-intensity imaging of gliomas in vivo.
Conclusions:
- AsT serves as a promising fluorescent probe for effective glioma imaging.
- The probe's ability to target glioma cells and cross the BBB facilitates improved visualization for surgical guidance.
- This development offers a potential advancement in neuro-oncology for enhanced glioma detection and treatment.
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