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Updated: Apr 30, 2026
Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells
Published on: September 25, 2016
Inverting microwell array chip for the cultivation of human induced pluripotent stem cells with controlled aggregate
Taku Satoh1, Shinji Sugiura1, Kimio Sumaru1
1Research Center for Stem Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), AIST Tsukuba Central 5, 1-1-1, Higashi, Tsukuba, Ibaraki 305-8568, Japan.
Abstract:
We present a novel cell culture chip, namely, "inverting microwell array chip," for cultivation of human induced pluripotent stem cells. The chip comprises a lower hydrogel microwell array and an upper polystyrene culture surface. We demonstrate the formation of uniform cellular aggregates in the microwell array, and after inversion, a culture with controlled aggregate size and geometrical arrangement on the polystyrene surface. Here, we report effects of cell concentrations on a cultivation sequence in the chip.
