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Updated: Apr 30, 2026
Multi-Stream Perfusion Bioreactor Integrated with Outlet Fractionation for Dynamic Cell Culture
Published on: July 20, 2022
A microfluidic perfusion platform for cultivation and screening study of motile microalgal cells
Young-Jae Eu1, Hye-Sun Park1, Dong-Pyo Kim2
1Department of Fine Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 305-764, South Korea ; National Center of Applied Microfluidic Chemistry, Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Gyungbuk 790-784, South Korea.
Abstract:
Systematic screening of algal cells is getting huge interest due to their capability of producing lipid-based biodiesel. Here, we introduce a new microfluidic platform composed of an array of perfusion chambers designed for long-term cultivation and preliminary screening of motile microalgal cells through loading and releasing of cells to and from the chambers. The chemical environment in each perfusion chamber was independently controlled for 5 days. The effect of nitrogen-depletion on the lipid production, phototaxis behavior in the absence of Ca(2+), and cytotoxic effect of herbicide on microalgal cells was successfully monitored and compared with simultaneous control experiments on the platform. The present methodology could be extended to effective screening of algal cells and various cell lines for the production of biodiesel and other useful chemicals.
