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Oxidative modification of proteins in pediatric cystic fibrosis with bacterial infections

Izabela Sadowska-Bartosz1, Sabina Galiniak1, Grzegorz Bartosz2

  • 1Department of Biochemistry and Cell Biology, University of Rzeszów, Zelwerowicza 4, 35-601 Rzeszów, Poland.

Oxidative Medicine and Cellular Longevity
|May 8, 2014
PubMed

Insights

Cystic fibrosis (CF) patients with chronic lung infections show increased oxidative stress markers. Glycophores and protein carbonyls are useful indicators of oxidative damage in CF patients.

Area of Science:

  • Biochemistry
  • Pulmonology
  • Medical Research

Background:

  • Chronic lung infections by Pseudomonas aeruginosa and Staphylococcus aureus are common in cystic fibrosis (CF) patients.
  • These infections induce significant oxidative stress in CF patients.
  • Understanding oxidative stress markers is crucial for managing CF.

Purpose of the Study:

  • To compare markers of plasma protein oxidative damage and glycoxidation in CF patients with chronic P. aeruginosa and S. aureus infections.
  • To assess erythrocyte antioxidant enzyme activities in these patients.
  • To investigate nitric oxide concentration in exhaled air of CF patients.

Main Methods:

  • Comparison of plasma protein oxidative damage and glycoxidation markers (glycophores, carbonyl groups, kynurenine).
  • Measurement of erythrocyte glutathione S-transferase activity.
  • Quantification of nitric oxide in exhaled air.

Main Results:

  • Elevated glycophore and protein carbonyl levels were observed in CF patients infected with P. aeruginosa and S. aureus compared to controls.
  • Increased glutathione S-transferase activity was noted in both infection groups.
  • Kynurenine levels were elevated specifically in S. aureus infected CF patients.

Conclusions:

  • These findings confirm the presence of oxidative stress in CF patients with chronic lung infections.
  • Glycophores and protein carbonyls serve as valuable markers for oxidative modifications of plasma proteins in CF.
  • Further research into antioxidant strategies for CF management is warranted.

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