Related Experiment Video
Updated: Apr 30, 2026

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs
Published on: January 27, 2021
Biological and functional properties of proteolytic enzyme-modified egg protein by-products
Marta Pokora1, Ewelina Eckert1, Aleksandra Zambrowicz1
1Department of Animal Products Technology and Quality Management, Wroclaw University of Environmental and Life Sciences Chełmonskiego 37/41, 51-630, Wrocław, Poland.
Abstract:
Enzymatic hydrolysis led to improve functional properties and biological activity of protein by-products, which can be further used as protein ingredients for food and feed applications. The effects of proteolytic enzyme modification of egg-yolk protein preparation (YP) and white protein preparation (WP), obtained as the by-products left during the course of lecithin, lysozyme, and cystatin isolation on their biological and functional properties, were evaluated by treating a commercial Neutrase. The antihypertensive and antioxidative properties of YP and WP hydrolysates were evaluated based on their angiotensin-converting enzyme (ACE)-inhibitory activity and radical scavenging (DPPH) capacity, ferric reducing power, and chelating of iron activity. The functionality of obtained hydrolysates was also determined. Neutrase caused a degree of hydrolysis (DH) of YP and WP by-products: 27.6% and 20.9%, respectively. In each of them, mixture of peptides with different molecular masses were also observed. YP hydrolysate showed high levels of antioxidant activity. The scavenging capacity, ferric reducing power, and chelating capacity were observed at the level: 0.44 μmol/L Trolox mg(-1), 177.35 μg Fe(2+) mg(-1), and 549.87 μg Fe(2+) mg(-1), respectively. YP hydrolysate also exhibited significant ACE-inhibitory activity, in which the level was 59.2 μg. Protein solubility was significantly improved as the DH increased. WP hydrolysate showed high water-holding capacity of 43.2. This study indicated that YP and WP hydrolysates could be used in foods as natural antioxidants and functionality enhancers.
Related Concept Videos
Protein Digestion
Protein Denaturation
The Proteasome Structure
The proteasome is an...
Mechanical Protein Function
Overview of Protein Metabolism
Amino acids play various roles in the body once they are absorbed into cells. They are restructured...

