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Related Experiment Videos

RNA editing in plant mitochondria.

R Hiesel1, B Wissinger, W Schuster

  • 1Institut für Genbiologische Forschung, Berlin, Federal Republic of Germany.

Science (New York, N.Y.)
|December 22, 1989
PubMed
Summary

Plant mitochondrial RNA editing modifies nucleotide sequences, altering codons to enhance amino acid conservation. This process, particularly the CGG to TGG conversion, suggests a common feature in higher plant mitochondria.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Plant Science

Background:

  • Comparative sequence analysis reveals differences between genomic DNA and messenger RNA (mRNA) in higher plants.
  • These divergences suggest post-transcriptional modifications occurring after transcription.

Purpose of the Study:

  • To investigate nucleotide sequence divergences between genomic DNA and mRNA in Oenothera mitochondria.
  • To determine the nature and implications of these sequence alterations, particularly in coding regions.

Main Methods:

  • Comparative sequence analysis of genomic DNA and complementary DNA (cDNA) clones.
  • Analysis of mitochondrial genes in the higher plant Oenothera.

Main Results:

  • Nucleotide sequence divergences were observed between genomic and mRNA-derived sequences.
  • Sequence alterations were explained by post-transcriptional nucleotide modifications.
  • Codon changes in mRNA specified evolutionarily conserved amino acids.
  • Genomic arginine codon CGG was edited to tryptophan codon TGG in mRNA at conserved positions.

Conclusions:

  • RNA editing in plant mitochondria uses the standard genetic code.
  • RNA editing explains the frequent occurrence of CGG codons and tryptophan.
  • RNA editing is likely a common and non-species-specific feature in all higher plant mitochondria.

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