Recent developments in liquid-phase separation techniques for metabolomics
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Updated: Apr 30, 2026

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
Rawi Ramautar1, Gerhardus J de Jong
1Analytical Biosciences, LACDR, Leiden University, PO Box 9502, 2300 RA, Leiden, The Netherlands.
Abstract:
Metabolomics is the comprehensive analysis of low molecular weight compounds in biological samples such as cells, body fluids and tissues. Comprehensive profiling of metabolites in complex sample matrices with the current analytical toolbox remains a huge challenge. Over the past few years, liquid chromatography-mass spectrometry (LC-MS) and capillary electrophoresis-mass spectrometry (CE-MS) have emerged as powerful complementary analytical techniques in the field of metabolomics. This Review provides an update of the most recent developments in LC-MS and CE-MS for metabolomics. Concerning LC-MS, attention is paid to developments in column technology and miniaturized systems, while strategies are discussed to improve the reproducibility and the concentration sensitivity of CE-MS for metabolomics studies. Novel interfacing techniques for coupling CE to MS are also considered. Representative examples illustrate the potential of the recent developments in LC-MS and CE-MS for metabolomics. Finally, some conclusions and perspectives are provided.
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