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Oxygenic Photosynthesis01:26

Oxygenic Photosynthesis

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Oxygenic photosynthesis is a fundamental process in which light energy is harnessed to drive the oxidation of water, leading to the production of molecular oxygen (O₂), adenosine triphosphate (ATP), and nicotinamide adenine dinucleotide phosphate (NADPH). This process is essential for sustaining aerobic life on Earth and is primarily carried out by cyanobacteria, algae, and plants. The core of oxygenic photosynthesis lies in the thylakoid membranes, where chlorophyll pigments facilitate...
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Photosystem II01:22

Photosystem II

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The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across  two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
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The Anatomy of Chloroplasts01:08

The Anatomy of Chloroplasts

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Green algae and plants, including green stems and unripe fruit, harbor specialized organelles called chloroplasts to carry out photosynthesis. They coordinate both stages of photosynthesis — the light-dependent reactions and the light-independent reactions. The light-dependent reactions use sunlight to release oxygen and produce chemical energy in the form of ATP and NADPH, and the light-independent reactions capture CO2 and use ATP and NADPH to produce sugar.
Structure of...
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Photosystems01:32

Photosystems

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Photosystems are multiprotein complexes that form the functional units of photosynthesis in plants, algae, and cyanobacteria. They are found embedded in the membrane of tiny sac-like structures called thylakoids placed inside the chloroplast.
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
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Anatomy of Chloroplasts01:07

Anatomy of Chloroplasts

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Green algae and plants, including green stems and unripe fruit, harbor chloroplasts—the vital organelles where photosynthesis takes place. In plants, the highest density of chloroplasts is found in the mesophyll cells of leaves.
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Anoxygenic Photosynthesis01:30

Anoxygenic Photosynthesis

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Anoxygenic photosynthesis is a phototrophic process that captures light energy to drive carbon fixation without producing molecular oxygen. Unlike oxygenic photosynthesis, which utilizes water as an electron donor and releases oxygen, anoxygenic phototrophs use alternative electron donors such as hydrogen sulfide (H₂S), elemental sulfur (S⁰), or thiosulfate (S₂O₃²⁻). This process is carried out by diverse groups of bacteria, including purple bacteria, green...
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Related Experiment Video

Updated: Apr 30, 2026

Author Spotlight: Extended Oxygen Consumption Measurement in Retinal Pigment Epithelium Using Resipher
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Author Spotlight: Extended Oxygen Consumption Measurement in Retinal Pigment Epithelium Using Resipher

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Oxygen concentration inside a functioning photosynthetic cell.

Shigeharu Kihara1, Daniel A Hartzler1, Sergei Savikhin1

  • 1Department of Physics, Purdue University, West Lafayette, Indiana.

Biophysical Journal
|May 9, 2014
PubMed
Summary

Early oxygenic photosynthetic bacteria likely evolved without reactive oxygen species protection. Mechanisms developed later as atmospheric oxygen rose, with higher concentrations found in colonies than solitary cells.

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Area of Science:

  • Biochemistry
  • Astrobiology
  • Evolutionary Biology

Background:

  • Oxygenic photosynthesis produces excess oxygen within cells.
  • Understanding intracellular oxygen levels is crucial for evolutionary studies.
  • Reactive oxygen species (ROS) pose a threat to cellular components.

Purpose of the Study:

  • Estimate excess oxygen concentration in photosynthetic membranes.
  • Investigate the implications for the evolution of early oxygenic photosynthesis.
  • Determine if colonial cyanobacteria require ROS protection.

Main Methods:

  • Applied classical diffusion theory.
  • Utilized experimental data on oxygen production rates.
  • Modeled excess oxygen concentrations in solitary cells and colonies.

Main Results:

  • Gloeobactor violaceus (plesiomorphic) had low excess oxygen (0.025 μM).
  • Synechocystis and Synechococcus (apomorphic) showed slightly higher levels (0.064–0.25 μM).
  • Colonies ≥40 μm exhibited excess oxygen comparable to air-saturated water.

Conclusions:

  • Early solitary photosynthetic bacteria likely evolved without ROS protection.
  • ROS protection mechanisms developed gradually with rising atmospheric oxygen.
  • Colonial cyanobacteria require ROS protection, even in anoxic environments.