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The Use of Cystometry in Small Rodents: A Study of Bladder Chemosensation
Published on: August 21, 2012
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Combination of bladder ultrasonography and novel cystometry method in mice reveals rapid decrease in bladder capacity
Kentaro Takezawa1, Makoto Kondo2, Hiroshi Kiuchi3
1Department of Neuroscience and Cell Biology, Osaka University Graduate School of Medicine, Osaka, Japan; and Department of Urology, Osaka University Graduate School of Medicine, Osaka, Japan.
American Journal of Physiology. Renal Physiology
|May 9, 2014
Summary
A new cystometry method using bladder ultrasonography in mice improves bladder function analysis. This technique enhances measurement of bladder capacity and compliance, aiding research into bladder dysfunction treatments.
Area of Science:
- Urology
- Animal Models
- Physiology
Background:
- In vivo cystometry is crucial for studying bladder dysfunction in animal models.
- Conventional cystometry in small animals, particularly mice, faces challenges with reliability and accuracy.
- Existing methods can be hindered by catheter placement, affecting bladder wall movement and measurement fidelity.
Purpose of the Study:
- To establish a novel, reliable cystometry method for evaluating mouse lower urinary tract function.
- To combine this new method with transabdominal bladder ultrasonography for comprehensive bladder analysis.
- To assess bladder functional changes in a lipopolysaccharide (LPS)-induced cystitis model.
Main Methods:
- Development of a new cystometry technique with anterior bladder wall catheter placement.
- Integration of transabdominal bladder ultrasonography with the novel cystometry procedure.
- Application of the combined method to analyze LPS-induced cystitis in mice.
Main Results:
- The novel method achieved better bladder distension, indicated by larger maximum bladder capacity, lower pressure thresholds, higher voided volumes, and improved bladder compliance.
- Ultrasonography proved useful for analyzing mouse bladder dynamics, maximum capacity, and post-voiding residual volume.
- LPS instillation caused rapid decreases in bladder capacity and voided volume, along with a significant reduction in bladder compliance not detectable by conventional methods.
Conclusions:
- The new anterior wall catheter cystometry combined with ultrasonography offers a more reliable and sensitive approach to mouse bladder function analysis.
- This combined method provides deeper insights into bladder dynamics and dysfunction, particularly in inflammatory conditions like LPS-induced cystitis.
- The technique holds potential as a powerful tool for advancing research and developing new treatments for bladder dysfunction.
