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Pathologic potential of variant clones of the oshima strain of far-eastern subtype tick-borne encephalitis virus

Le Xuan Luat1, Mya Myat Ngwe Tun1, Corazon C Buerano1

  • 1Department of Virology, Institute of Tropical Medicine, Nagasaki University.

Tropical Medicine and Health
|May 9, 2014
PubMed

Insights

Tick-borne encephalitis virus (TBEV) variants were isolated and studied. An E protein substitution influenced TBEV

Area of Science:

  • Virology
  • Immunology
  • Pathology

Background:

  • Tick-borne encephalitis virus (TBEV) causes severe central nervous system (CNS) disease.
  • Previous research suggested TBEV infection and immune response contribute to mortality.
  • The role of TBEV variants in pathogenicity was not previously examined.

Purpose of the Study:

  • To investigate the biological and pathological potential of TBEV variant clones from the Oshima strain.
  • To identify genetic differences contributing to variant characteristics.
  • To understand the impact of specific mutations on TBEV pathogenicity.

Main Methods:

  • Isolation and characterization of eight TBEV variant clones from the Oshima 5-10 strain.
  • Assessment of plaque morphology in BHK cells and pathogenicity in mice.
  • Full genome sequencing of viral variants to identify genetic alterations.

Main Results:

  • Isolated TBEV variants displayed distinct plaque morphologies and pathogenic potentials in mice.
  • Genetic analysis revealed specific nucleotide and amino acid changes in most variants compared to the parent strain.
  • An E protein substitution (Glu122→Gly) was associated with altered in vivo infection, replication, and reduced mouse pathogenicity.

Conclusions:

  • The TBEV Oshima strain harbors diverse variants (quasispecies).
  • Specific genetic mutations, like the E protein substitution, significantly impact TBEV's biological behavior and virulence.
  • Understanding variant-specific immune responses is crucial for developing effective TBEV vaccines and treatments.
Keywords:
E proteinFar-eastern subtype TBEVvariant clones

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