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Further evidence for coelomic-associated B lymphocytes
M A Marcos1, F Huetz, P Pereira
1Unité d'Immunobiologie, Institut Pasteur, Paris, France.
European Journal of Immunology
|November 1, 1989
Summary
Researchers discovered a distinct B cell population in the pleuropericardial cavity, characterized by high IgM and low IgD. These "coelomic" B cells may originate locally, differentiating from intraembryonic precursors.
Area of Science:
- Immunology
- Developmental Biology
- Cell Biology
Background:
- CD5 B lymphocytes are typically found in gut-associated lymphoid tissues.
- Previous studies noted their presence in mesenchymal areas like peritoneum and tonsils.
Purpose of the Study:
- To characterize a unique B cell population identified in the pleuropericardial spaces.
- To investigate the origin and phenotype of these novel B cells.
Main Methods:
- Flow cytometry analysis of B cell populations in mouse pleuropericardial cavities.
- Phenotypic characterization including surface marker expression (IgM, IgD, Mac-1, B220, CD5).
- Analysis of antibody clonotypes and comparison with known B cell subsets.
Main Results:
- A homogeneous population of large, noncycling, nonsecretory B cells was found in pleuropericardial spaces.
- These cells exhibit high surface IgM, low IgD, Mac-1 expression, and low B220 levels.
- While phenotypically similar to CD5 B cells, many were CD5-negative across mouse strains.
Conclusions:
- The pleuropericardial cavity harbors a distinct B cell population, proposed as "coelomic" B cells.
- These cells likely differentiate locally from intraembryonic precursors within the coelomic cavity.
- This finding distinguishes them from conventional bone marrow-derived "stromal" B cells.