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In silico analysis of expression pattern of a Wnt/β-catenin responsive gene ANLN in gastric cancer
Narayanan Sathiya Pandi1, Muthaiah Manimuthu1, Palaniswamy Harunipriya1
1Unit of Rural Biotechnology, Saraswathi Narayanan College, Madurai Kamaraj University, Madurai 625022, India.
Abstract:
Actin-binding protein anillin (ANLN) is primarily involved in the cytokinesis and known to be dysregulated in many cancers including gastric cancer (GC). However, the regulation and clinical significance of ANLN in GC are far less clear. In the present study, we aimed to investigate the clinical significance and possible regulators of ANLN in GC. We have identified the Wnt/β-catenin associated regulation of ANLN by analyzing the in vitro perturbed β-catenin mRNA expression profiles. Investigating the gastric tumors from publicly available genome-wide mRNA expression profiles, we have identified the over expression of ANLN in gastric tumors. Association between ANLN expression and clinical characteristics of GC showed elevated expression in intestinal type GC. Performing a single sample prediction method across GC mRNA expression profiles, we have identified the over expression of ANLN in proliferative type gastric tumors compared to the invasive and metabolic type gastric tumors. In silico pathway prediction analysis revealed the association between Wnt/β-catenin signaling and ANLN expression in gastric tumors. Our results highlight that expression of a Wnt/β-catenin responsive gene ANLN in GC is a molecular predictor of intestinal and proliferative type gastric tumors.
Insights
Anillin (ANLN) is overexpressed in gastric cancer (GC), particularly in intestinal and proliferative types. Its expression is linked to Wnt/β-catenin signaling, suggesting ANLN as a potential biomarker for gastric cancer subtypes.
Area of Science:
- Oncology
- Molecular Biology
- Genomics
Background:
- Anillin (ANLN), an actin-binding protein, plays a role in cytokinesis and is dysregulated in various cancers, including gastric cancer (GC).
- The specific regulatory mechanisms and clinical implications of ANLN in GC remain largely unelucidated.
Purpose of the Study:
- To investigate the clinical significance of ANLN in GC.
- To identify potential regulators of ANLN expression in GC.
- To explore the association between ANLN and GC subtypes.
Main Methods:
- Analysis of in vitro perturbed β-catenin mRNA expression profiles to identify Wnt/β-catenin associated regulation.
- Examination of publicly available genome-wide mRNA expression profiles from gastric tumors.
- Application of single-sample prediction methods and in silico pathway analysis.
Main Results:
- ANLN is significantly overexpressed in gastric tumors compared to normal tissue.
- Elevated ANLN expression correlates with the intestinal subtype of GC.
- ANLN is overexpressed in proliferative type GC tumors relative to invasive and metabolic types.
- In silico analysis confirmed a link between Wnt/β-catenin signaling and ANLN expression in GC.
Conclusions:
- ANLN expression serves as a molecular predictor for intestinal and proliferative subtypes of gastric cancer.
- The Wnt/β-catenin pathway is implicated in the regulation of ANLN in GC.
- Understanding ANLN's role offers insights into GC heterogeneity and potential therapeutic targets.
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