Development of morin-conjugated Au nanoparticles: exploring the interaction efficiency with BSA using spectroscopic...

Area of Science:

• Nanotechnology
• Biochemistry
• Materials Science

Background:

• Morin is an antioxidant and anti-tumor drug.
• Biomacromolecule interaction efficiency is crucial for therapeutic agents.
• Nanoparticle conjugation can enhance drug properties.

Purpose of the Study:

• To conjugate morin with citrate-coated gold nanoparticles (M-C-AuNPs).
• To investigate the interaction between M-C-AuNPs and bovine serum albumin (BSA).
• To evaluate the effect of M-C-AuNPs on morin-BSA binding affinity.

Main Methods:

• Synthesis of M-C-AuNPs via reduction of chloroauric acid.
• Characterization using UV-vis spectroscopy, TEM, XRD, and FTIR.
• Spectroscopic investigation of M-C-AuNPs and BSA interactions using UV-vis, fluorescence, and CD spectroscopy.

Main Results:

• M-C-AuNPs were successfully synthesized and characterized.
• Spectroscopic analysis revealed interactions between M-C-AuNPs and BSA.
• M-C-AuNPs significantly enhanced the binding force between morin and BSA compared to unconjugated morin.

Conclusions:

• M-C-AuNPs exhibit improved binding affinity with BSA.
• The conjugation of morin with gold nanoparticles enhances its interaction with biomacromolecules.
• This study provides a foundation for designing nanotherapeutic agents with enhanced functionality.