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Acute mental stress, induced by public speaking, increased salivary cortisol, protein, and pH levels in male students. However, stress did not significantly alter salivary fluoride concentration in either supernatant saliva or sediment.

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Area of Science:

  • Oral Health
  • Biochemistry
  • Psychoneuroendocrinology

Background:

  • Acute mental stress can impact physiological parameters.
  • Saliva composition, including fluoride, protein, and pH, may be affected by stress.
  • Understanding these variations is crucial for oral health research.

Purpose of the Study:

  • To assess stress-related variations in salivary fluoride, cortisol, total protein, and pH.
  • To investigate the influence of acute mental stress on these salivary components.
  • To test the hypothesis that stress reactions do not influence these parameters.

Main Methods:

  • Thirty-four male students participated in a crossover study design.
  • Acute mental stress was induced via public speaking followed by tooth brushing.
  • Saliva samples were collected pre-stress, post-stress, and at multiple time points after tooth brushing.
  • Measurements included salivary cortisol, total protein, pH, and fluoride concentration in supernatant and sediment.

Main Results:

  • Salivary cortisol concentration significantly increased 20 minutes post-stress.
  • Salivary protein concentration and pH also showed significant increases after public speaking.
  • No significant alterations in salivary fluoride concentration (supernatant or sediment) were observed within 2 hours post-stress.
  • Fluoride bioavailability was higher in salivary sediment compared to supernatant saliva.

Conclusions:

  • Acute mental stress, such as public speaking, significantly affects salivary cortisol, protein, and pH levels.
  • Despite these changes, stress does not appear to alter salivary fluoride concentration in the short term.
  • Salivary sediment plays a role in fluoride bioavailability.