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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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RNA Interference01:23

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Types of RNA01:23

Types of RNA

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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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Viruses with RNA Genomes01:29

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RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
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Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

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Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
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siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

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Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
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Identification of Circular RNAs using RNA Sequencing
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Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

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Detecting and characterizing circular RNAs.

William R Jeck1, Norman E Sharpless2

  • 1Department of Genetics, University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA.

Nature Biotechnology
|May 10, 2014
PubMed
Summary
This summary is machine-generated.

Circular RNAs, once difficult to study, are now being comprehensively analyzed. Emerging research suggests these circular RNA molecules play regulatory roles with potential therapeutic applications.

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Identification of Circular RNAs using RNA Sequencing
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Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Circular RNAs (circRNAs) were discovered in the 1990s but remained poorly understood due to analytical challenges.
  • Traditional RNA analysis methods hindered comprehensive study of circRNA species.
  • Recent advancements have enabled deeper investigation into these molecules.

Purpose of the Study:

  • To explore the comprehensive analysis of circular RNA species.
  • To understand the potential regulatory functions and therapeutic implications of circRNAs.
  • To identify future research challenges in the study of circRNAs.

Main Methods:

  • Utilized novel bioinformatic approaches.
  • Employed biochemical enrichment strategies.
  • Leveraged deep sequencing technologies for comprehensive studies.

Main Results:

  • Thousands of endogenous circular RNAs identified in mammalian cells.
  • Some circRNAs are highly abundant and evolutionarily conserved.
  • Emerging evidence suggests circRNAs regulate microRNA (miRNA) function and transcriptional control.

Conclusions:

  • Circular RNAs represent a significant class of noncoding RNAs with potential research and therapeutic applications.
  • Understanding the regulation and function of circRNAs is a key future challenge.
  • Advanced methodologies have unlocked comprehensive studies of circRNAs.