Related Experiment Video
Updated: Apr 30, 2026
Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
Published on: April 29, 2015
[Effect of paeoniflorin on secretion of TGF-beta1 from macrophages in mice]
Objective:
To explore the effect of paeoniflorin (PAE) on the production of transforming growth factor beta1 (TGF-beta1) from peritoneal macrophages(PMs) stimulated by soluble egg antigen (SEA) of Schistosoma japonicum.
Methods:
SEA was prepared by trituration and added into culture plank, flask and dish containing PMs which were cultured for 24 h. TGF-beta1 secreted from PMs was measured by ELISA. TGF-beta1 mRNA and protein produced from PMs were evaluated by RT-PCR and Western blotting, respectively. SEA (10 mg/L) 5 ml was added into culture flask and dish containing PMs. PMs were cultured for 12 h, and PAE at different concentrations (0, 7.5, 15, 30, 60, 120 mg/L) was added into the culture flask and dish, and PMs were cultured consecutively for another 12 h and 24 h, respectively. TGF-beta1 mRNA and protein from PMs stimulated by SEA were evaluated by RT-PCR and Western blotting, respectively.
Results:
TGF-beta1 (235.86 +/- 3.43 ng/L) was produced from PMs under stimulation of SEA at 10 mg/L, and the expression of TGF-beta1 mRNA and protein in PMs were depressed significantly by PAE in a concentration-dependent manner (r = -0.827, P < 0.01; r = -0.952, P < 0.01, respectively).
Conclusion:
PAE inhibits the production of TGF-beta1 from PMs stimulated by SEA.
Insights
Paeoniflorin (PAE) significantly reduces transforming growth factor beta1 (TGF-beta1) production in macrophages stimulated by Schistosoma japonicum soluble egg antigen (SEA). This study demonstrates PAE
Area of Science:
- Immunology
- Pharmacology
- Parasitology
Context:
- Schistosoma japonicum infection triggers significant immune responses.
- Peritoneal macrophages (PMs) play a crucial role in the inflammatory process.
- Transforming growth factor beta1 (TGF-beta1) is a key cytokine involved in immune regulation.
Purpose:
- To investigate the inhibitory effect of paeoniflorin (PAE) on TGF-beta1 production.
- To determine the impact of PAE on TGF-beta1 expression in PMs stimulated by soluble egg antigen (SEA).
Summary:
- Peritoneal macrophages were stimulated with SEA, and TGF-beta1 production was measured.
- PAE was administered at various concentrations to assess its effect on TGF-beta1.
- ELISA, RT-PCR, and Western blotting confirmed that PAE significantly inhibited TGF-beta1 mRNA and protein expression in a dose-dependent manner.
Impact:
- Paeoniflorin demonstrates potential as a therapeutic agent to modulate immune responses in schistosomiasis.
- Findings suggest PAE could be explored for managing TGF-beta1-mediated pathologies associated with parasitic infections.
More Related Videos
11:48Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
Published on: May 31, 2018
08:34Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages
Published on: February 22, 2017