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Updated: Apr 30, 2026
Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
Published on: July 17, 2021
[Construction and expression of recombinant baculovirus with Schistosoma japonicum SjPP gene]
Objective:
To express the soluble recombinant Schistosoma japonicum SjPP proteins in TN5B1-4 cells.
Methods:
The total RNA was extracted from adult worms of Schistosoma japonicum. The whole coding sequence of SjPP gene was synthesized by RT-PCR and cloned into donor plasmid. The recombinant donor pFastBac-SjPP was transformed into E.coli DH10Bac forming Bacmid-SjPP which was transfected into insect cell with cational lipofectin. The fusion protein SjPP was analyzed with SDS-PAGE and Western blotting.
Results:
The infective recombinant baculovirus Bacmid-SjPP was obtained and SjPP protein was expressed in insect cells.
Conclusion:
The recombinant protein SjPP has been expressed in insect TN5B1-4 cells with proper antigenicity.
