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Immunohistochemical expression of cyclooxygenase-2 in normal, hyperplastic and neoplastic canine lymphoid tissues
P Asproni1, M Vignoli2, S Cancedda2
1Dipartimento di Scienze Veterinarie, University of Pisa, Pisa I-56124, Italy.
Abstract:
There is much interest in the potential use of selective inhibitors of cyclooxygenase (COX)-2 in combination with other cancer therapeutics. COX-2 is a key enzyme in prostaglandin synthesis and has been implicated in the pathogenesis of numerous canine and feline malignancies. There are few data on the potential role of COX-2 in the pathogenesis of canine lymphoma. The present study examined COX-2 expression in normal, hyperplastic and neoplastic canine lymphoid tissues. Immunohistochemical expression was evaluated in 12 samples of non-pathologically enlarged normal lymph nodes, 24 samples of hyperplastic lymph node and 44 samples of lymphoma (22 B-cell and 22 T-cell lymphomas). The labelling was scored semiquantitatively and a score of +2 or +3 was interpreted as overexpression. In hyperplastic lymph nodes only a few macrophages were COX-2-positive while six of the 44 lymphomas (13.6%; three B- and three T-cell lymphomas) overexpressed COX-2. These data provide a rationale for further investigation of COX-2 expression in canine lymphoma for prognostic, chemopreventive and chemotherapeutic purposes.
Insights
Cyclooxygenase (COX)-2 is investigated in canine lymphoma. Overexpression of COX-2 was found in 13.6% of lymphomas, suggesting its potential role in cancer therapy.
Area of Science:
- Veterinary Oncology
- Immunohistochemistry
- Molecular Biology
Background:
- Cyclooxygenase (COX)-2 is implicated in various canine and feline cancers.
- Limited data exist on COX-2's role in canine lymphoma pathogenesis.
- Selective COX-2 inhibitors are of interest for combined cancer therapy.
Purpose of the Study:
- To examine cyclooxygenase (COX)-2 expression in normal, hyperplastic, and neoplastic canine lymphoid tissues.
- To assess the potential role of COX-2 in canine lymphoma development.
- To provide a rationale for further research into COX-2 in canine lymphoma.
Main Methods:
- Immunohistochemistry was used to evaluate COX-2 expression.
- Samples included normal lymph nodes (12), hyperplastic lymph nodes (24), and lymphomas (44; 22 B-cell, 22 T-cell).
- COX-2 labeling was scored semiquantitatively; scores of +2 or +3 indicated overexpression.
Main Results:
- Few COX-2-positive macrophages were observed in hyperplastic lymph nodes.
- Six out of 44 canine lymphomas (13.6%) showed COX-2 overexpression.
- Overexpression was observed in three B-cell and three T-cell lymphomas.
Conclusions:
- COX-2 is overexpressed in a subset of canine lymphomas.
- These findings support further investigation of COX-2 in canine lymphoma.
- Potential applications include prognostic, chemopreventive, and chemotherapeutic strategies.
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