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Necrotic regions are absent in fiber-shaped cell aggregates, approximately 100 μm in diameter
Takayuki Takei1, Jyunpei Kitazono1, Sadao Tanaka2
1a Department of Chemical Engineering , Graduate School of Science and Engineering, Kagoshima University , Korimoto, Kagoshima , Japan.
Abstract:
Microscopic, fiber-shaped cell aggregates, have been used as building blocks for fabricating macroscopic three-dimensional tissue architectures, in the field of tissue engineering. In this study, we examined the occurrence of necrotic regions in the most widely used, fiber-shaped cell aggregates, approximately 100 μm in diameter. Alginate hydrogel hollow microfibers were used as templates for the cell aggregates. We demonstrated negligible necrotic region formation occurred in the cell aggregates formed in the hollow microfibers. Furthermore, we improved on previously-reported methods for preparing the hollow microfibers to avoid common microfiber tangling during the fiber preparation process.
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