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Related Concept Videos

Regulated mRNA Transport02:22

Regulated mRNA Transport

5.7K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
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Regulated mRNA Transport02:22

Regulated mRNA Transport

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Nuclear Export of mRNA02:31

Nuclear Export of mRNA

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Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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Nuclear Export of mRNA02:31

Nuclear Export of mRNA

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Nuclear Export01:42

Nuclear Export

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The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
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mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

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The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
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Related Experiment Video

Updated: Apr 30, 2026

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
11:32

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Published on: December 4, 2010

17.1K

Mechanisms of mRNA export.

Petra Björk1, Lars Wieslander1

  • 1Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, SE-106 91 Stockholm, Sweden.

Seminars in Cell & Developmental Biology
|May 13, 2014
PubMed
Summary

This review covers essential steps in eukaryotic gene expression, focusing on messenger ribonucleoprotein particle (mRNP) processing, movement, and export from the nucleus to the cytoplasm.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Eukaryotic gene expression involves intricate steps for accurate protein synthesis.
  • Messenger ribonucleoprotein particles (mRNPs) are key intermediates in this process.

Purpose of the Study:

  • To review the intranuclear stages of gene expression.
  • To detail the journey of mRNPs from transcription to nuclear export.

Main Methods:

  • Literature review of existing research on mRNP biogenesis and trafficking.
  • Analysis of key molecular interactions and pathways involved.

Main Results:

  • mRNPs must be correctly processed and assembled for efficient gene expression.
  • The movement of mRNPs through the interchromatin is crucial for their function.
Keywords:
Gene expressionPre-mRNA processingmRNAmRNP export

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  • Interaction with Nuclear Pore Complexes (NPCs) mediates the export of mRNPs.

    • Conclusions:

    • Successful mRNP release, transport, and NPC interaction are vital for eukaryotic gene expression.
    • Understanding these intranuclear steps is fundamental to comprehending gene regulation.