Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Targeting Lcn2 to Inhibit Myocardial Cell Ferroptosis is a Potential Therapy for Alleviating Septic Cardiomyopathy.

Inflammation·2025

Same author

Development and validation of a screening tool for sepsis without laboratory results in the emergency department: a machine learning study.

EClinicalMedicine·2025

Same author

UPLC-MS metabolite profiling and antioxidant activity of <i>Sanghuangporus sanghuang</i> extract.

PeerJ·2025

Same author

Mechanical properties and mechanisms of soda residue and fly ash stabilized soil.

Scientific reports·2025

Same author

Angelica gigas Nakai (Korean Dang-gui) Root Alcoholic Extracts in Health Promotion and Disease Therapy - active Phytochemicals and In Vivo Molecular Targets.

Pharmaceutical research·2025

Same author

Extension of an ICU-based noninvasive model to predict latent shock in the emergency department: an exploratory study.

Frontiers in cardiovascular medicine·2025

Search research articles

Related Experiment Video

Updated: Apr 30, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

A simple, sensitive colorimetric assay for coralyne based on target induced split G-quadruplex formation.

Yingya Kan¹, Cheng Jiang, Qiang Xi

¹State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry

|May 13, 2014

Summary

Researchers developed a simple colorimetric assay to detect coralyne, a polydeoxyadenosine (poly(dA))-binding drug. This method utilizes target-induced split G-quadruplex formation for efficient drug screening.

More Related Videos

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Related Experiment Videos

Last Updated: Apr 30, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

Area of Science:

Biochemistry
Molecular Biology
Drug Discovery

Background:

Screening for drugs that bind to polydeoxyadenosine (poly(dA)) is crucial for developing new therapies.
Poly(dA)-binding drugs, such as coralyne, show significant antitumor activity.
Developing efficient and simple detection methods for these drugs is essential.

Purpose of the Study:

To develop a straightforward colorimetric strategy for detecting coralyne, a poly(dA)-binding drug.
To leverage target-induced split G-quadruplex formation for drug detection.
To establish a cost-effective and visible assay for potential drug screening applications.

Main Methods:

Utilized two DNA oligonucleotides containing split G-quadruplex and adenine-rich sequences.
Coralyne binding to poly(dA) induced the formation of a split G-quadruplex DNAzyme.
The DNAzyme catalyzed the generation of a colored product for colorimetric detection.

Main Results:

The developed assay successfully detected coralyne through a colorimetric response.
The assay demonstrated a linear detection range from 0.033 to 1.667 μM.
A low detection limit of 16 nM was achieved, indicating high sensitivity.

Conclusions:

A simple, cost-effective, and visible colorimetric assay for coralyne detection was successfully developed.
The assay utilizes target-induced split G-quadruplex formation, offering a novel detection mechanism.
This method shows great potential for application in high-throughput drug screening for poly(dA)-binding agents.