Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ion Exchange01:17

Ion Exchange

1.6K
Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
1.6K
Complexation Equilibria: The Chelate Effect01:19

Complexation Equilibria: The Chelate Effect

1.7K
In complexation reactions, metal atoms or cations interact with ligands to form donor-acceptor adducts called metal complexes. Ligands that bind through one donor site are monodentate, ligands with two donor sites are bidentate, and those with more than two donor sites are polydentate ligands. For example, ethylene diamine is a bidentate ligand that binds through two nitrogen donor atoms, forming a five-membered ring. EDTA is a polydentate ligand that binds through four oxygen and two nitrogen...
1.7K
Noncovalent Attractions in Biomolecules02:35

Noncovalent Attractions in Biomolecules

27.9K
Noncovalent attractions are associations within and between molecules that influence the shape and structural stability of complexes. These interactions differ from covalent bonding in that they do not involve sharing of electrons.
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
27.9K
Noncovalent Attractions in Biomolecules02:35

Noncovalent Attractions in Biomolecules

19.8K
19.8K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.1K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.1K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

1.0K
1.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photoactivatable BODIPY Dyes Conjugated to Self-Labeling Protein Tags.

ACS applied bio materials·2026
Same author

Photoinduced Inactivation of Pathogenic Microorganisms via Cotton Textile Functionalized with a Novel Iodinated BODIPY Derivative.

Molecules (Basel, Switzerland)·2026
Same author

Nanostructured Lipid Carriers Containing Acridine Derivatives in the Application of Sonodynamic Therapy for the Treatment of Breast Cancer.

ACS omega·2026
Same author

A BODIPY-Hoechst conjugate for nuclear DNA staining.

Journal of porphyrins and phthalocyanines·2026
Same author

Paclitaxel and rose bengal loaded microbubbles for the ultrasound targeted chemo-sonodynamic therapy of pancreatic cancer.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2025
Same author

Design of halloysite nanotube-based nanomaterials for theranostic applications: fluorescent probes and chemodynamic activity.

Journal of materials chemistry. B·2025

Related Experiment Video

Updated: Apr 30, 2026

Heterogeneous Removal of Water-Soluble Ruthenium Olefin Metathesis Catalyst from Aqueous Media Via Host-Guest Interaction
10:39

Heterogeneous Removal of Water-Soluble Ruthenium Olefin Metathesis Catalyst from Aqueous Media Via Host-Guest Interaction

Published on: August 23, 2018

7.2K

Intracellular guest exchange between dynamic supramolecular hosts.

Subramani Swaminathan1, Colin Fowley, Bridgeen McCaughan

  • 1Laboratory for Molecular Photonics, Department of Chemistry, University of Miami , 1301 Memorial Drive, Coral Gables, Florida 33146-0431, United States.

Journal of the American Chemical Society
|May 13, 2014
PubMed
Summary

Amphiphilic polymers self-assemble into nanoparticles that efficiently capture and transfer energy between molecular donors and acceptors. These dynamic nanocarriers can deliver and co-localize cargo within cancer cells for targeted intracellular energy transfer.

More Related Videos

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags
10:10

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

Published on: January 17, 2019

10.3K
Synthesis and Characterization of Supramolecular Colloids
09:26

Synthesis and Characterization of Supramolecular Colloids

Published on: April 22, 2016

9.5K

Related Experiment Videos

Last Updated: Apr 30, 2026

Heterogeneous Removal of Water-Soluble Ruthenium Olefin Metathesis Catalyst from Aqueous Media Via Host-Guest Interaction
10:39

Heterogeneous Removal of Water-Soluble Ruthenium Olefin Metathesis Catalyst from Aqueous Media Via Host-Guest Interaction

Published on: August 23, 2018

7.2K
Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags
10:10

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

Published on: January 17, 2019

10.3K
Synthesis and Characterization of Supramolecular Colloids
09:26

Synthesis and Characterization of Supramolecular Colloids

Published on: April 22, 2016

9.5K

Area of Science:

  • Polymer Chemistry
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Amphiphilic polymers are crucial for developing novel drug delivery systems.
  • Self-assembling nanoparticles offer controlled environments for molecular interactions.
  • Efficient intracellular delivery and cargo co-localization remain challenges in nanomedicine.

Purpose of the Study:

  • To synthesize and characterize amphiphilic polymers capable of forming energy-transferring nanoparticles.
  • To investigate the cargo loading, energy transfer efficiency, and dynamic exchange properties of these nanoparticles.
  • To evaluate the potential of these nanocarriers for intracellular delivery and targeted energy transfer in cancer cells.

Main Methods:

  • Synthesis of poly(methacrylate) backbone with decyl and oligo(ethylene glycol) chains.
  • Nanoparticle self-assembly in aqueous buffer and characterization of hydrodynamic diameter.
  • Loading of anthracene donors and borondipyrromethene acceptors into nanoparticle hydrophobic cores.
  • Förster Resonance Energy Transfer (FRET) efficiency measurements.
  • Cell culture studies involving cervical cancer cells and nanoparticle incubation for intracellular delivery and energy transfer assessment.

Main Results:

  • Amphiphilic polymers self-assembled into 15 nm nanoparticles with a hydrophobic-to-hydrophilic ratio of 2.5.
  • Nanoparticles achieved 95% excitation energy transfer efficiency between encapsulated donors and acceptors.
  • Supramolecular assemblies demonstrated rapid guest exchange in aqueous media, enabling co-localization of chromophores.
  • Nanoparticles successfully crossed the membrane of cervical cancer cells, facilitating intracellular co-localization and energy transfer.
  • Sequential incubation strategies allowed for cargo exchange and energy transfer exclusively within the intracellular environment.

Conclusions:

  • Dynamic amphiphilic polymer nanoparticles can efficiently encapsulate and co-localize molecular guests.
  • These nanocarriers facilitate targeted intracellular energy transfer by enabling cargo exchange within the cellular environment.
  • The ability to independently deliver and trigger interactions of species within cells opens new avenues for theranostics.