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Updated: Apr 30, 2026

In Vitro Synthesis of Modified mRNA for Induction of Protein Expression in Human Cells
Published on: November 13, 2014
Synthetic mRNAs for manipulating cellular phenotypes: an overview
Elgar Susanne Quabius1, Guido Krupp2
1Department of Otorhinolaryngology, Head and Neck Surgery, Christian-Albrechts-University Kiel, Arnold-Heller-Str. 3, Building 27, D-24105 Kiel, Germany; Institute of Immunology, Christian-Albrechts-University Kiel, Arnold-Heller-Str. 3, Building 17, D-24105 Kiel, Germany.
Abstract:
Availability of high quality synthetic mRNAs (syn-mRNAs) has enabled progress in their applications. Important structural features and quality requirements are discussed. Developments in the application of mRNA-mediated manipulation of cells are presented (i) mRNA-directed expression of antigens in dendritic cells for vaccination projects in oncogenesis, infectious disease and allergy prevention; (ii) reprogramming of human fibroblasts to induced pluripotent stem cells with their subsequent differentiation to the desired cell type; (iii) applications in gene therapy.
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