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Updated: Apr 30, 2026
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Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
Published on: July 16, 2019
11.2K
TILLING for plant breeding
1Plant Breeding Institute, University of Sydney, Private Bag 4011, Narellan, NSW, 2567, Australia.
Methods in Molecular Biology (Clifton, N.J.)
|May 13, 2014
Summary
We describe a simple, low-cost TILLING method combining mutagenesis and SNP detection for isolating gene mutations. This technique aids plant functional genomics and breeding by generating useful genetic variation.
Area of Science:
- Plant functional genomics
- Molecular biology
- Plant breeding
Background:
- Targeting Induced Local Lesions IN Genomes (TILLING) is a key method in plant functional genomics.
- It combines mutagenesis with mutation detection for gene analysis.
- TILLING also serves as a valuable tool in plant breeding for generating genetic variation.
Purpose of the Study:
- To present a straightforward and economical TILLING procedure.
- To facilitate the isolation of mutations in genes of interest.
- To support plant breeding programs through targeted genetic variation.
Main Methods:
- Utilizes a combination of chemical mutagenesis and Single Nucleotide Polymorphism (SNP) detection.
- Focuses on a simplified, low-cost approach to TILLING.
- Applicable to identifying variations in known or candidate genes.
Main Results:
- Successfully outlines a simple and cost-effective TILLING protocol.
- Enables the isolation of specific mutations within genes.
- Provides a method for generating desired genetic variation in plants.
Conclusions:
- The described TILLING procedure is accessible and economical.
- It effectively supports research in plant functional genomics.
- This method offers practical applications for plant breeding initiatives.
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