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Updated: Apr 30, 2026

Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
Published on: February 14, 2020
Agrobacterium-mediated transformation of barley (Hordeum vulgare L.)
Ainur Ismagul1, Iryna Mazonka, Corinne Callegari
1Australian Centre for Plant Functional Genomics, University of Adelaide, PMB 1, Glen Osmond, SA, Australia.
Abstract:
Barley biotechnology requires efficient genetic engineering tools for producing transgenic plants necessary for conducting reverse genetics analyses in breeding and functional genomics research. Agrobacterium-mediated genetic transformation is an important technique for producing barley transgenics with simple low-copy number transgenes. This chapter reports a refined protocol for the systematic high-throughput transformation of the advanced Australian spring barley breeding line WI4330.
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