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Updated: Apr 30, 2026
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 25, 2010
Expression of trehalose synthase gene from Pseudomonas putida P06 in Pichia pastoris
Wang Tengfei1, Hongjuan Liu2, Kun Dai2
1Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, TEDA, Tianjin, PR China / Key Laboratory of Shandong Microbial Engineering, QILU University of Technology, Jinan, Shandong, PR China.
Abstract:
The trehalose synthase (TreS) gene from Pseudomonas putida P06 was successfully ligated with pPICZaA expression vector by the EcoRI and XbaI and was transformed into Pichia pastoris GS115 by electrotransformation. The trehalose synthase gene was fused to the genome of Pichia pastoris GS115 and was controlled by AOX1 promoter. The TreS protein was successfully expressed in intracellularly. SDS-PAGE results illustrated that a specificity protein band was observed at about 76 kDa. The cell lysates could convert 60% maltose into trehalose at 50 °C and pH 7.5 in 10% maltose substrate for 24 h. The Pichia pastorisas exogenous gene expression host is safer to produce endotoxin free TreS than E.coli.
