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Trop2 gene: a novel target for cervical cancer treatment
Xiaoqi Liu1, Siqi Li, Faping Yi
1Department of Biochemistry and Molecular Biology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing, 400016, China.
Object:
Trop2 plays an important role in proliferation and invasion of tumors. Extensive research has shown that the expression level of Trop2 is closely related to the progress of cervical diseases. This study was to explore the effects of Trop2 on cell proliferation and apoptosis in cervical cancer.
Methods:
Trop2 was knocked down by shRNA in CaSki cells. The expression level of mRNA and protein was detected by real-time PCR and western blot, respectively. Cell proliferation was determined by CCK-8 and clone formation assay; apoptosis was measured by flow cytometry; cell cycle and apoptosis-related proteins cyclinD1, P53, bcl-2, bax, caspase 3, 8 and 9 were analyzed as well to investigate possible mechanism.
Results:
Trop2 expression was effectively repressed in CaSki cells by Trop2 shRNA, which resulted in inhibition of proliferation and colony formation, whereas apoptosis rate was significantly increased. Furthermore, in Trop2 knockdown CaSki cells, the expression of cyclinD1 and bcl-2 was significantly down-regulated, while that of P53 and bax was up-regulated accompanied by increased activities of caspase 9 and 3 but not caspase 8.
Conclusions:
Trop2 is important in proliferation and apoptosis regulation in CaSki cells, which may become a novel target for cervical cancer treatment.
Insights
Trop2 knockdown inhibited cervical cancer cell proliferation and colony formation while increasing apoptosis. This suggests Trop2 is a potential therapeutic target for cervical cancer.
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Trop2 (Trophoblast cell surface antigen 2) is implicated in tumor proliferation and invasion.
- Trop2 expression correlates with the progression of cervical diseases.
Purpose of the Study:
- To investigate the role of Trop2 in regulating cell proliferation and apoptosis in cervical cancer.
- To explore Trop2's impact on cervical cancer cell behavior.
Main Methods:
- Trop2 was downregulated using shRNA in CaSki cervical cancer cells.
- Cell proliferation, apoptosis, cell cycle, and related protein expression (cyclinD1, P53, bcl-2, bax, caspase 3, 8, 9) were analyzed.
Main Results:
- Trop2 knockdown significantly inhibited CaSki cell proliferation and colony formation.
- Apoptosis rate increased, with altered expression of key regulatory proteins (downregulated cyclinD1, bcl-2; upregulated P53, bax) and activated caspases 3 and 9.
Conclusions:
- Trop2 plays a critical role in regulating proliferation and apoptosis in cervical cancer cells.
- Trop2 represents a promising novel therapeutic target for cervical cancer treatment.
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