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Studies on non-specific interference due to serum in the avidin-biotin microELISA for monkey chorionic gonadotropin
1Department of Biochemistry, Indian Institute of Science, Bangalore.
Journal of Immunological Methods
|December 20, 1989
Abstract:
A study has been carried out on the non-specific interference due to serum in the avidin biotin microELISA for monkey chorionic gonadotropin. Results suggest that it is not due to any proteolytic activity in the serum, but immunoglobulin or associated factors interfering at the level of antigen-antibody interaction. This interference was eliminated by heating samples at 60 degrees C for 30 min.