Related Experiment Video

Updated: Apr 30, 2026

Ferric Chloride-induced Murine Thrombosis Models
10:37

Ferric Chloride-induced Murine Thrombosis Models

Published on: September 5, 2016

21.9K

Antithrombotic activities of sulforaphane via inhibiting platelet aggregation and FIIa/FXa

Sae-Kwang Ku1, Jong-Sup Bae

  • 1Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University, Gyeongsan, 712-715, Republic of Korea.

Archives of Pharmacal Research
|May 13, 2014
PubMed

Insights

Sulforaphane (SFN), found in cruciferous vegetables, demonstrates significant anticoagulant and antithrombotic effects. This natural compound prolongs clotting times and inhibits key coagulation factors, suggesting potential for novel anticoagulant therapies.

Area of Science:

  • Biochemistry
  • Pharmacology
  • Hematology

Background:

  • Sulforaphane (SFN) is a natural isothiocyanate found in cruciferous vegetables.
  • SFN has demonstrated efficacy in preventing carcinogenesis, diabetes, and inflammatory responses.

Purpose of the Study:

  • To investigate the anticoagulant activities of Sulforaphane (SFN).
  • To evaluate the effects of SFN on coagulation factors and fibrinolysis in vitro and in vivo.

Main Methods:

  • Assessed SFN's impact on activated partial thromboplastin time (aPTT) and prothrombin time (PT).
  • Monitored thrombin (Factor IIa, FIIa) and activated factor X (FXa) activities and production in human umbilical vein endothelial cells (HUVECs).
  • Evaluated SFN's effects on plasminogen activator inhibitor type 1 (PAI-1) and tissue-type plasminogen activator (t-PA) expression in TNF-α activated HUVECs.

Main Results:

  • SFN treatment prolonged aPTT and PT, and inhibited thrombin and FXa activities and production.
  • SFN inhibited thrombin-catalyzed fibrin polymerization and platelet aggregation.
  • SFN demonstrated anticoagulant effects in mice and reduced the PAI-1 to t-PA ratio.

Conclusions:

  • Sulforaphane (SFN) exhibits significant antithrombotic activities.
  • SFN's anticoagulant properties provide a foundation for developing novel anticoagulant agents.

More Related Videos

Author Spotlight: Innovative Techniques for ROS Detection and Implications for Platelet Research06:35

Author Spotlight: Innovative Techniques for ROS Detection and Implications for Platelet Research

Published on: March 29, 2024

1.4K
Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells13:38

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells

Published on: January 18, 2017

9.7K

Related Concept Videos

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors01:20

Antiplatelet Drugs: Prostaglandin Synthesis, P2Y12 and Glycoprotein IIb/IIIa Inhibitors

Antiplatelet drugs emerge as frontline defenders against the insidious threat of thromboembolic diseases, where abnormal clots obstruct vital blood vessels. These drugs stand as bulwarks, inhibiting platelet aggregation and clot formation, thereby mitigating the risk of life-threatening conditions like myocardial infarction, coronary artery disease, and thrombotic strokes.
Prostaglandin synthesis inhibitors, exemplified by the widely known aspirin, wield their power by irreversibly acetylating...
1.6K
Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
2.6K
Anticoagulant Drugs: Vitamin K Antagonists and Direct Oral Anticoagulants01:18

Anticoagulant Drugs: Vitamin K Antagonists and Direct Oral Anticoagulants

Oral anticoagulants are vital tools in preventing and treating blood clotting disorders. This diverse class of medications can be categorized as vitamin K antagonists, exemplified by warfarin, and direct thrombin inhibitors (DTIs), such as dabigatran, as well as factor Xa inhibitors, including rivaroxaban.
Warfarin, a prominent vitamin K antagonist family member, exerts its effect by inhibiting the enzyme VKORC1 (vitamin K epoxide reductase complex 1). By hindering this enzyme, warfarin...
2.7K
Clot Retraction and Fibrinolysis01:16

Clot Retraction and Fibrinolysis

After a fibrin clot is formed, the next step is clot retraction, a vital process facilitated by platelet contractile proteins, such as actin and myosin. These proteins pull the fibrin strands closer together and condense the clot. This action reduces the size of the clot, creating a smaller, denser structure that effectively seals off the damaged vessel. Clot retraction consolidates the clot and helps with wound healing by bringing the edges of the damaged blood vessel closer together.
6.0K
Venous Thrombosis III: Interprofessional Care01:29

Venous Thrombosis III: Interprofessional Care

Venous thrombosis requires effective prevention and treatment strategies to improve patient outcomes and reduce potential complications.Prevention StrategiesHealthcare providers must prioritize preventing venous thromboembolism (VTE) for all adult patients upon admission. Interventions depend on bleeding and thrombosis risk, medical history, current medications, diagnoses, planned procedures, and patient preferences. Patients on bed rest should change positions every two hours and, if not...
482
Formation of the Platelet Plug01:22

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
6.2K