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Staphylococcus pseudintermedius for CAMP-test.

Vincenzo Savini1, Antonello Paparella2, Annalisa Serio2

  • 1Clinical Microbiology and Virology, Spirito Santo Hospital Pescara (PE), Italy.

International Journal of Clinical and Experimental Pathology
|May 13, 2014
PubMed
Summary
This summary is machine-generated.

The CAMP test detects Listeria monocytogenes and Streptococcus agalactiae using Staphylococcus aureus. Staphylococcus pseudintermedius, a related bacteria, can also be used, offering a reliable alternative for bacterial identification.

Keywords:
CAMP-testGBSListeria monocytogenesStaphylococcus pseudintermediusβ-hemolysin

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Area of Science:

  • Microbiology
  • Bacteriology
  • Diagnostic Microbiology

Background:

  • The CAMP test is a standard method for identifying *Listeria monocytogenes* (Lm) and *Streptococcus agalactiae* (group B streptococcus, GBS).
  • This test relies on the synergistic hemolysis produced when these bacteria grow near a beta-hemolysin-producing *Staphylococcus aureus* (Sa) strain.
  • Beta-hemolysin production in *Staphylococcus aureus* is variable and strain-dependent.

Purpose of the Study:

  • To evaluate *Staphylococcus pseudintermedius* (Sp) as a potential alternative to *Staphylococcus aureus* in the CAMP test.
  • To determine if constitutive beta-hemolysin production by Sp can support the characteristic CAMP reaction with Lm and GBS.

Main Methods:

  • The CAMP test was performed by streaking isolates of Lm and GBS perpendicularly to reference strains of Sa and clinical isolates of Sp.
  • Hemolytic zones were observed and characterized, specifically looking for the typical arrow-shaped hemolysis indicative of a positive reaction.
  • The constitutive beta-hemolysin production of Sp was confirmed.

Main Results:

  • Both Lm and GBS produced characteristic arrow-shaped hemolytic zones when incubated with Sp.
  • Sp demonstrated constitutive beta-hemolysin production across all tested clinical isolates, unlike the strain-dependent production in Sa.
  • The results indicate Sp can effectively facilitate the CAMP reaction.

Conclusions:

  • *Staphylococcus pseudintermedius* is a viable and reliable alternative to *Staphylococcus aureus* for performing the CAMP test.
  • The constitutive hemolytic activity of Sp simplifies the test by removing the need for specific beta-hemolysin-producing reference strains.
  • This finding has implications for routine bacterial identification in clinical and veterinary microbiology settings.