Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conjugation01:19

Conjugation

3.0K
Conjugation is a form of horizontal gene transfer that primarily occurs in bacteria and some archaea, promoting genetic diversity and adaptation. Bacteria can acquire resistance genes through conjugative plasmids, allowing them to survive antibiotic treatments that would otherwise be lethal. This process involves direct contact between cells through specialized structures such as the sex pilus and is mediated by conjugative plasmids, including the F (fertility) factor.Conjugation requires...
3.0K
Mechanism of Conjugation01:19

Mechanism of Conjugation

1.6K
Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...
1.6K
Transduction01:16

Transduction

3.0K
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
3.0K
Types of Genetic Transfer Between Organisms02:18

Types of Genetic Transfer Between Organisms

24.8K
Genetic transfer occurs when genetic information is passed from one organism to another. It occurs via two mechanisms: vertical gene transfer and horizontal gene transfer. Vertical gene transfer occurs when genetic information is transferred from one generation to the next, which happens much more frequently than horizontal gene transfer. Both sexual and asexual reproduction are forms of vertical gene transfer, where one or more organisms pass some or all of their genome onto their progeny.
24.8K
Types of Genetic Transfer Between Organisms02:18

Types of Genetic Transfer Between Organisms

5.7K
5.7K
Bacterial Transformation01:33

Bacterial Transformation

9.1K
9.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

"Molecular insights into the bactericidal toxin Tle1 of Pseudomonas aeruginosa: Interaction with VgrG, its adaptor, and its immunity protein".

The Journal of biological chemistry·2026
Same author

Artificial symbiont replacement in a vertically transmitted plant symbiosis reveals a role for microbe-microbe interactions in enforcing specificity.

The ISME journal·2025
Same author

Uncovering a new family of conserved virulence factors that promote the production of host-damaging outer membrane vesicles in gram-negative bacteria.

Journal of extracellular vesicles·2025
Same author

Structures and roles of BcsD and partner scaffold proteins in proteobacterial cellulose secretion.

Current biology : CB·2023
Same author

What Is New in the Anti-<i>Pseudomonas aeruginosa</i> Clinical Development Pipeline Since the 2017 WHO Alert?

Frontiers in cellular and infection microbiology·2022
Same author

Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms.

Nature communications·2021

Related Experiment Video

Updated: Apr 30, 2026

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
08:25

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Published on: October 31, 2019

16.1K

Gene transfer: conjugation.

Thibault G Sana1, Aurélie Laubier, Sophie Bleves

  • 1Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR7255-CNRS & Aix-Marseille Université, IMM, 31 chemin Jospeh Aiguier, 13402, Marseille, France.

Methods in Molecular Biology (Clifton, N.J.)
|May 14, 2014
PubMed
Summary

Bacterial conjugation is a gene transfer method where bacteria share DNA through direct cell contact. This process, mediated by plasmids, facilitates genetic exchange between microorganisms.

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Gene transfer is crucial for bacterial evolution and adaptation.
  • Conjugation is a primary mechanism for horizontal gene transfer in bacteria.
  • Plasmids and transposons are key mobile genetic elements mediating conjugation.

Purpose of the Study:

  • To describe the process of plasmid conjugation.
  • To elucidate the mechanism of DNA transfer during bacterial conjugation.

Main Methods:

  • Detailed description of the plasmid conjugation process.
  • Explanation of the role of conjugative pili in DNA transfer.

Main Results:

  • Plasmid conjugation involves direct cell-to-cell contact between donor and recipient bacteria.

More Related Videos

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
10:41

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

Published on: January 4, 2017

13.0K
Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
11:36

Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

Published on: September 23, 2017

17.5K

Related Experiment Videos

Last Updated: Apr 30, 2026

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit
08:25

Genetic Modification of Cyanobacteria by Conjugation Using the CyanoGate Modular Cloning Toolkit

Published on: October 31, 2019

16.1K
Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation
10:41

Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

Published on: January 4, 2017

13.0K
Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
11:36

Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

Published on: September 23, 2017

17.5K
  • DNA transfer is facilitated by specialized structures called conjugative pili.

    • Conclusions:

    • Plasmid conjugation is a fundamental process for genetic exchange in bacterial populations.
    • Understanding conjugation is vital for fields such as antibiotic resistance and biotechnology.