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Related Concept Videos

Proteomics01:33

Proteomics

7.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.5K

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Related Experiment Video

Updated: Apr 30, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

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Proteomic analysis.

Pascal Cosette1, Thierry Jouenne

  • 1"Polymères, Biopolymères, Surfaces" Laboratory, UMR CNRS 6270, PISSARO Proteomic Facility, Institute for Research and Innovation in Biomedicine, University of Rouen, 76821, Mont-Saint-Aignan, cedex, France.

Methods in Molecular Biology (Clifton, N.J.)
|May 14, 2014
PubMed
Summary

This chapter details methods for assessing the Pseudomonas aeruginosa proteome using two-dimensional gel electrophoresis. Robust experimental design is crucial for reproducible proteomic analysis of bacterial proteins.

Area of Science:

  • Proteomics
  • Microbiology
  • Biochemistry

Background:

  • Proteomics offers insights into protein quantity, modifications, and localization.
  • Accurate proteomic analysis requires robust experimental design for reproducibility.
  • Pseudomonas aeruginosa is a significant bacterial pathogen requiring detailed molecular study.

Purpose of the Study:

  • To provide detailed methods for assessing the proteome of Pseudomonas aeruginosa.
  • To outline a reproducible two-dimensional gel electrophoresis (2D-GE) approach for bacterial proteome analysis.
  • To guide researchers in applying proteomic tools for biological questions.

Main Methods:

  • Two-dimensional gel electrophoresis (2D-GE) for protein separation.
  • Immobilized pH gradients (IPG) for high-resolution isoelectric focusing.

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  • Liquid Chromatography coupled with tandem Mass Spectrometry (LC-MS/MS) for protein identification.

    • Main Results:

    • Established protocols for crude protein extraction from Pseudomonas aeruginosa.
    • Optimized methods for protein separation using IPG-based 2D-GE.
    • Demonstrated feasibility of LC-MS/MS for identifying separated bacterial proteins.

    Conclusions:

    • The described 2D-GE approach provides a robust method for Pseudomonas aeruginosa proteome assessment.
    • Reproducible proteomic analysis is achievable with meticulous experimental design and standardized protocols.
    • This methodology facilitates deeper understanding of bacterial protein expression and regulation.