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Updated: Aug 14, 2026

Capture Compound Mass Spectrometry - A Powerful Tool to Identify Novel c-di-GMP Effector Proteins
Published on: March 29, 2015
LC/MS/MS-based quantitative assay for the secondary messenger molecule, c-di-GMP
Yasuhiko Irie1, Matthew R Parsek
1Centre for Biomolecular Sciences, School of Molecular Medical Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
Abstract:
The secondary messenger molecule, 3',5'-cyclic diguanosine monophosphate (c-di-GMP), controls various cellular processes in bacteria. Direct measurement of intracellular concentration of c-di-GMP is fast becoming an important tool for studying prokaryotic biology. Here, we describe a comprehensive extraction protocol from live bacteria and quantitative analysis using LC/MS/MS.
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