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Updated: Apr 30, 2026

Cell-cell Fusion of Genome Edited Cell Lines for Perturbation of Cellular Structure and Function
Published on: December 7, 2019
Chromosomal integration of transcriptional fusions
Rafael Silva-Rocha1, Víctor de Lorenzo
1Faculdade de Medicina, University of Sao Paulo, Ribeirão Preto, SP, Brazil.
Abstract:
The characterization and parameterization of promoters is crucial for the study of gene regulatory networks. While a number of techniques are available for this purpose, the use of reporter fusions integrated in the chromosome of a bacterial host affords precise quantification of transcriptional responses with high reproducibility. Here, we describe the integration of green fluorescent protein (GFP) and lacZ reporter cassettes using either mini-Tn7-based vectors or homologous chromosomal recombination to analyze gene regulation at transcriptional and post-transcriptional levels.
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