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Related Concept Videos

DNA-only Transposons02:57

DNA-only Transposons

15.9K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
15.9K
Transposons01:24

Transposons

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Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
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Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

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Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
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In-vitro Mutagenesis01:16

In-vitro Mutagenesis

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.8K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

12.4K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
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In vitro Mutagenesis01:16

In vitro Mutagenesis

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Related Experiment Video

Updated: Apr 30, 2026

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
13:31

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

Published on: October 31, 2014

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Transposon mutagenesis.

Hemantha D Kulasekara1

  • 1Department of Microbiology, University of Washington, 1959 NE Pacific St HSB K161, Seattle, WA, 98195, USA, donhk@uw.edu.

Methods in Molecular Biology (Clifton, N.J.)
|May 14, 2014
PubMed
Summary

This study details a Himar1 mariner-based transposon mutagenesis protocol for Pseudomonas aeruginosa. This method efficiently generates mutant libraries to identify genes controlling specific bacterial phenotypes.

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Transposon-based mutagenesis is crucial for identifying genes controlling bacterial phenotypes.
  • Himar1 mariner and Tn5 transposases are commonly used for generating mutant libraries in Pseudomonas aeruginosa.

Purpose of the Study:

  • To present a detailed protocol for Himar1 mariner-based transposon mutagenesis in P. aeruginosa.
  • To facilitate the creation of comprehensive mutant libraries for genetic studies.

Main Methods:

  • Utilizing Himar1 mariner transposon system for mutagenesis.
  • Application of the protocol to Pseudomonas aeruginosa.
  • Generation of nonredundant mutant libraries.

Main Results:

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Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
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Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

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Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
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Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

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Related Experiment Videos

Last Updated: Apr 30, 2026

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
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Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis

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Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
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Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri

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Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
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Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

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  • A detailed, reproducible protocol for Himar1 mariner-based transposon mutagenesis in P. aeruginosa is provided.
  • The method enables the construction of extensive mutant libraries.

Conclusions:

  • Himar1 mariner transposon mutagenesis offers a robust approach for genetic analysis in P. aeruginosa.
  • This protocol serves as a valuable resource for researchers studying bacterial genetics and phenotypes.