Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Burn mouse models.

Henrik Calum1, Niels Høiby, Claus Moser

  • 1Department of Clinical Microbiology, University Hospital of Copenhagen, Rigshospitalet, Copenhagen, Denmark.

Methods in Molecular Biology (Clifton, N.J.)
|May 14, 2014
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

IMPACT-Introduction of Mycotic Prophylaxis At Cystectomy Trial: perioperative mycotic prophylaxis with fluconazole to reduce postoperative complications following cystectomy-study protocol for a national multi-center, double-blinded, placebo-controlled, randomized clinical trial.

Trials·2026
Same author

Patients with obesity have a higher risk of periprosthetic joint infection and more frequent polymicrobial infections after knee arthroplasty: A nationwide register-based study from Denmark.

Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA·2026
Same author

Urinary Secretory-IgA and IgG Responses and Inflammatory Markers in Patients With Pseudomonas aeruginosa Urinary Tract Infections.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·2026
Same author

Hyperbaric Oxygen Treatment as a Novel Add-on in Selected Patients with Infective Endocarditis - A Safety and Feasibility Trial.

Infection and drug resistance·2026
Same author

Prosthetic Valve Endocarditis-Insights from the NatIonal Danish Endocarditis stUdieS (NIDUS) Registry.

Diagnostics (Basel, Switzerland)·2026
Same author

A design of experiments study of nasal spray deposition in the alberta idealized nasal inlet.

Drug delivery and translational research·2026

Severe thermal injury impairs the immune system, leading to reduced white blood cells and increased bacterial infection susceptibility in burn wound mice. This new model aids in developing treatments for burn victims.

Area of Science:

  • Immunology
  • Wound Healing
  • Infectious Disease

Background:

  • Severe thermal injuries cause significant immunosuppression, affecting the entire immune system.
  • This immunosuppression is particularly pronounced when large body surface areas are affected.
  • Existing animal models may not fully capture the complexity of burn-induced immune dysfunction.

Purpose of the Study:

  • To establish and characterize a novel mouse model of severe thermal injury.
  • To investigate the impact of burn injury on peripheral blood leukocyte concentrations.
  • To evaluate the susceptibility to bacterial infection and associated inflammatory responses in the burn model.

Main Methods:

  • A 6% third-degree burn was induced in mice using a hot-air blower, with histological confirmation.

Related Experiment Videos

  • Peripheral blood was analyzed at 48 hours post-burn to assess leukocyte counts.
  • Mice were infected with Pseudomonas aeruginosa to evaluate bacterial dissemination and inflammatory responses.

    • Main Results:

    • Burned mice showed a significant decline in peripheral blood leukocytes, primarily polymorphonuclear neutrophils and monocytes.
    • Pseudomonas aeruginosa infection led to bacterial dissemination exclusively in the burn wound group.
    • Histological analysis revealed increased neutrophil-dominated inflammation in infected burn wounds compared to non-infected burns.

    Conclusions:

    • The developed mouse model accurately reflects clinical burn injury scenarios.
    • The model demonstrates burn-induced immunosuppression and increased susceptibility to bacterial infection.
    • This model is valuable for testing new therapeutic strategies, including antibiotics and immunotherapies, for burn wound management.