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Isolation and gut microbiota modulation of antibiotic-resistant probiotics from human feces

Peng Tian1, Bo Xu1, Hanxiao Sun1

  • 1Institute of Genomic Medicine, College of Pharmacy, Jinan University, Guangzhou 510632, China.

Diagnostic Microbiology and Infectious Disease
|May 14, 2014
PubMed

Insights

Antibiotic-resistant probiotics can restore gut microbiota balance after antibiotic treatment. This study isolated resistant bacteria, showing they effectively improved gut health in mice, validating bacteriotherapy.

Area of Science:

  • Microbiology
  • Gastroenterology
  • Probiotics Research

Background:

  • Antibiotic use can disrupt the gut microbiota, leading to imbalance.
  • Probiotics offer a potential therapeutic strategy to restore gut homeostasis.
  • Antibiotic-resistant probiotics may be particularly beneficial in this context.

Purpose of the Study:

  • To isolate antibiotic-resistant bacteria from human fecal samples for potential probiotic use.
  • To evaluate the efficacy of these isolates in ameliorating antibiotic-induced gut microbiota imbalance in a mouse model.
  • To validate bacteriotherapy as a treatment for gut dysbiosis.

Main Methods:

  • Isolation of antibiotic-resistant bacteria from fecal samples of healthy adults and infants.
  • Establishment of a gut microbiota imbalance mouse model using antibiotic treatment.
  • Administration of a probiotic cocktail derived from isolated bacteria to the model mice.
  • Assessment of fecal microbiota composition, diversity, and gut health indicators (diarrhea, mucosal edema).

Main Results:

  • Eight antibiotic-resistant bacterial isolates were identified, with six formulated into a probiotic cocktail.
  • Probiotic treatment significantly increased the abundance of anaerobes, lactobacilli, and Bifidobacterium compared to the model group.
  • Gut microbiota richness and diversity indices were significantly higher in the probiotic-treated group.
  • Symptoms such as diarrhea and mucosal edema were alleviated in the probiotic group.

Conclusions:

  • Antibiotic-resistant bacteria can be effectively isolated and utilized as probiotics.
  • Bacteriotherapy using these probiotics demonstrates efficacy in restoring gut microbiota balance and alleviating antibiotic-induced dysbiosis.
  • The findings support the therapeutic potential of antibiotic-resistant probiotics for managing gut microbiota imbalances.
Keywords:
BacteriotherapyDiarrheaGut microbiota imbalanceProbiotics cocktail

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