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A vector that expresses secreted proteins on the cell surface.

X B Wang¹, R Milne, Y Marcel

¹Laboratory of Lipoprotein Metabolism, Institut de Recherches Cliniques de Montreal, Quebec, Canada.

DNA (Mary Ann Liebert, Inc.)

|December 1, 1989

Summary

A novel CDM8PI vector enables cell surface expression of secreted proteins via fusion with LFA-3. This expands epitope mapping techniques to secreted proteins and facilitates recovery of mutated proteins.

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Area of Science:

Molecular Biology
Protein Engineering
Immunology

Background:

The epitope-loss mutant isolation technique is crucial for mapping protein epitopes.
Existing methods are limited in their application to secreted proteins.
The CDM8 vector is a foundational tool for epitope mapping.

Purpose of the Study:

To construct a new expression vector, CDM8PI, for enhanced protein analysis.
To enable cell surface display of secreted proteins through fusion.
To extend the utility of epitope-loss mutant isolation to secreted proteins.

Main Methods:

Construction of the CDM8PI vector, derived from CDM8.
Generation of fusion proteins linking secreted proteins with the LFA-3 membrane anchor.

Related Experiment Videos

Expression of fusion proteins on the cell surface.

Main Results:

The CDM8PI vector successfully facilitates the production and cell surface expression of fusion proteins.
The vector enables the application of epitope-loss mutant isolation to secreted proteins.
Mutated proteins can be easily recovered in unfused forms post-selection.

Conclusions:

CDM8PI is a versatile vector for studying secreted proteins.
The new vector expands the scope of epitope mapping techniques.
Facilitates characterization of secreted protein mutations and epitopes.