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Updated: Apr 29, 2026

Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
Published on: October 15, 2018
A versatile methodology for the regioselective C⁸-metalation of purine bases
Dirk Brackemeyer1, Alexandre Hervé, Christian Schulte to Brinke
1Institut für Anorganische und Analytische Chemie, Westfälische Wilhelms-Universität Münster , D-48149 Münster, Germany.
Abstract:
Purine nucleobases are excellent ligands for metal ions, forming normally coordinative Werner-type bonds by utilizing the N donor atoms of the nucleobase skeleton. Here we show that purines such as 8-chlorocaffeine and 8-bromo-9-methyladenine react with [Pt(PPh3)4] under oxidative addition of the C(8)-halogen bond to the metal center. The resulting Pt(II) complexes feature a C(8)-bound ylidene ligand. Protonation of the ylidene at the N(7/9)-atom yields complexes bearing a protic N-heterocyclic carbene ligand derived from the purine base with an NMe,NH-substitution pattern.
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