Related Experiment Video
Updated: Apr 29, 2026
Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains
Published on: January 5, 2016
Molecular analysis of α-synuclein gene in Parkinson's disease in North Karnataka, India
G S Kadakol, S S Kulkarni, G M Wali
1Karntaka Institute for DNA Research, Pavate Nagar, Dharwad, Karnataka, India.
Background:
Parkinson's disease (PD) is a disabling neurological disorder characterized by progressive degeneration of dopaminergic neurons. Mutations analysis within the α-synuclein gene (SNCA) on chromosome 4 has been reported in the last decade.
Objective:
To elucidate the possible role of SNCA gene in the pathogenesis of PD in Indian population specifically in north Karnataka.
Materials And Methods:
The study subjects included 100 clinically diagnosed PD patients and 100 ethnically matched healthy controls. Isolated deoxyribonucleic acid (DNA) samples from both were subjected to exon-specific polymerase chain reaction (PCR) amplification and amplicons were subjected to capillary-based direct DNA sequencing.
Result:
No mutations were observed in SNCA gene of PD samples in comparison with control samples.
Conclusion:
These findings support the hypothesis that the SNCA gene mutations might be population specific and may not be playing role in causing PD in all the populations.
More Related Videos
12:01Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein
Published on: May 30, 2015
14:45Analyzing the Parkinson's Disease Mouse Model Induced by Adeno-associated Viral Vectors Encoding Human α-Synuclein
Published on: July 29, 2022
Related Concept Videos
01:24Parkinson Disease ll: Pathophysiology
01:24Parkinson Disease l: Introduction
01:37Neural Regulation