Related Experiment Video
Updated: Apr 29, 2026
11:22
Analysis of Gene Expression in Emerald Ash Borer Agrilus planipennis Using Quantitative Real Time-PCR
Published on: May 4, 2010
9.9K
Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative
Hui Ling1, Qibin Wu1, Jinlong Guo1
1Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
Plos One
|May 15, 2014
Summary
This study identifies the most stable reference genes for accurate gene expression analysis in sugarcane using qRT-PCR. GAPDH, eEF-1a, and eIF-4α are recommended for normalization across various samples.
Area of Science:
- Plant molecular biology
- Gene expression analysis
Background:
- Real-time quantitative reverse transcription-PCR (qRT-PCR) is crucial for gene expression analysis.
- Accurate normalization requires stable reference genes, which are underexplored in sugarcane (Saccharum officinarum).
Purpose of the Study:
- To systematically evaluate the expression stability of 13 candidate reference genes in sugarcane.
- To identify the most suitable reference genes for accurate gene expression quantification in sugarcane.
Main Methods:
- Evaluated 13 candidate reference genes using geNorm, NormFinder, and deltaCt methods.
- Assessed gene stability across different sugarcane tissues and experimental treatments.
Main Results:
- GAPDH, eEF-1a, and eIF-4α demonstrated the highest stability across all tested samples.
- APRT showed potential for studying gene copy number and transcript levels.
- Specific gene combinations were recommended for normalization under different experimental conditions (hormone treatment, abiotic stress, cultivar tissues).
Conclusions:
- This study provides the first systematic validation of reference genes for transcript expression profiling in sugarcane.
- The findings will aid researchers in selecting appropriate reference genes for precise gene expression quantification in sugarcane and other plant species.
