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MicroRNA-146a expression and its intervention in NF-кB signaling pathway in diabetic rat aorta
Objective:
The present study was designed to evaluate whether microRNA-146a, as an NF-кB regulating factor and its adapter proteins (TRAF6 and IRAK1), are affected by diabetes in rat aorta.
Methods:
Male Wistar rats were randomly divided into control and diabetic groups (n=6 in each). Diabetes was induced by a single injection of streptozotocin (55 mg/kg; i.p.) in 12 h fasted rats. The gene expression of microRNA-146a, NF-κB, IRAK1, and TRAF6 were determined by real time PCR.
Results:
The expression of microRNA-146a was down-regulated in diabetic aorta when compared with the control group (p<0.05). The mRNA expression levels of NF-кB, TRAF6 and IRAK1 also increased in the diabetic rat aorta when compared with their control counterparts (p<0.01 for all comparisons).
Conclusion:
These results suggest that down-regulation of microRNA-146a may lead to derangement in NF-кB negative feedback loop, propelling the aorta toward inflammation.
Insights
Diabetes down-regulates microRNA-146a in rat aorta, potentially causing inflammation by disrupting the NF-κB feedback loop. This affects key adapter proteins TRAF6 and IRAK1.
Area of Science:
- Cardiovascular Research
- Molecular Biology
- Endocrinology
Background:
- Diabetes mellitus is a significant risk factor for cardiovascular complications.
- The role of microRNAs in diabetic vascular dysfunction is an emerging area of research.
- NF-κB signaling pathway is implicated in vascular inflammation.
Purpose of the Study:
- To investigate the expression of microRNA-146a in the aorta of diabetic rats.
- To determine the impact of diabetes on NF-κB, TRAF6, and IRAK1 expression in rat aorta.
- To explore the relationship between microRNA-146a and NF-κB pathway components in diabetic vasculopathy.
Main Methods:
- Male Wistar rats were induced with diabetes using streptozotocin.
- Gene expression of microRNA-146a, NF-κB, IRAK1, and TRAF6 was quantified using real-time PCR.
- Control and diabetic groups were compared for molecular changes in aortic tissue.
Main Results:
- MicroRNA-146a expression was significantly down-regulated in diabetic rat aortas.
- mRNA levels of NF-κB, TRAF6, and IRAK1 were significantly elevated in diabetic aortas.
- A negative correlation between microRNA-146a and NF-κB pathway components was observed.
Conclusions:
- Down-regulation of microRNA-146a may disrupt the NF-κB negative feedback loop in diabetic aorta.
- This disruption potentially contributes to aortic inflammation in diabetes.
- MicroRNA-146a is a potential regulator of inflammatory processes in diabetic cardiovascular disease.
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